The selection of both appropriate positive and negative controls is important to evaluate biological experiments. There are a series of non-specific binding contribute to the background straining levels associated with primary antibodies, which include Fc receptors, reactive epitopes, endogenous enzymes, and other off-target effects. In order to reduce the risk of false-positive results, isotype controls are used to confirm that the primary antibody binding is specific.
In general, isotype controls are raised against antigens not found in common preclinical species so that lacks specific target binding. The isotype control antibody should closely match the properties of the primary antibody, including host species, isotype, class, working concentration, and conjugation.
The isotype control antibodies produced in Creative Biolabs can be used in different experiments, such as flow cytometry (FC), immunohistochemistry (IHC), enzyme-linked immunosorbent assay (ELISA), western blotting (WB), and immunocytochemistry (ICC).
Loading controls are high-expression proteins in the cell type you are interested in. Loading controls have been widely used in the Western blotting experiment. Loading controls are essential to normalize the levels of protein detection by ensuring that the protein-loaded equally across all gels. The selection of the appropriate loading control antibody is largely determined by the target molecular weight, sample subcellular fraction, and the cell/tissue types.
Creative Biolabs now can provide a variety of high-quality isotype & loading control antibodies with various conjugations. If you are interested in our products, please do not hesitate to contact us for more details.
