Secondary antibodies are monoclonal or polyclonal antibodies bind to primary antibodies or antibody fragments. Secondary antibodies provide signal detection and amplification along with extending the utility of an antibody through conjugation to proteins. Secondary antibodies can be conjugated to enzymes, fluorescent dyes, or other molecules, which include but not limited to horseradish peroxidase (HRP), alkaline phosphatase (AP), fluorescein isothiocyanate (FITC), rhodamine derivatives, Alexa Fluor dyes. In biological research, secondary antibodies have been widely used in various biochemical assays, such as Western blot, immunostaining, immunohistochemistry, immunocytochemistry.
Compared with the direct detection using primary antibodies, the indirect detection of secondary antibodies presents increased sensitivity, diversity, flexibility, and versatility. In addition, secondary antibodies bind to the heavy chains of primary antibodies so that don’t interfere with the binding between primary antibodies and antigens.
Recombinant proteins are frequently expressed along with a tag epitope, a small antigenic sequence recognized specifically by anti-tag antibodies. The most common tags include HA, His, Myc, GST, GFP, mCherry, etc. Creative Biolabs provides antibodies against a wide range of these tags. These anti-tag antibodies present high sensitivity and specificity that can be used to purify and detect recombinant proteins with low expression levels.
Based on the class of the primary antibody, the source host, downstream application, as well as the tag epitope, suitable secondary & tag antibodies can be selected for your experiment requirement. Creative Biolabs now can provide a variety of high-quality secondary & tag antibodies with various conjugations. If you are interested in our products, please do not hesitate to contact us for more details.
