Anti-Glycan Antibodies in Glycan Purification

Anti-glycan antibodies are antibodies to specific glycan epitopes and thus can bind specifically to glycoproteins containing these determinants. Due to the specificity of anti-glycan antibodies, they are used as essential tools for basic research. Here, Creative Biolabs gives an introduction to the application of anti-glycan antibodies in glycan/glycoprotein purification.

Use of Anti-Glycan Antibodies in Glycan Purification

Glycan purification is a necessary step for glycosylation analysis of glycoproteins. Approaches such as affinity chromatography or affinity binding and immunoprecipitation can be used in glycan purification. Antibodies can be noncovalently captured on protein A (or G)-Sepharose and then used to isolate glycoconjugates expressing specific glycan determinants. For instance, Falck et al. (2017) reported a high-throughput workflow for analysis of erythropoietin (EPO) glycosylation, which consists of (1) EPO affinity purification, based on Sepharose bead-linked anti-EPO antibodies, from cell culture supernatant (CCS), (2) efficiently stabilizing and easily interpretable sialic acid derivation, (3) straightforward and easily automatable sample preparation, (4) high sensitivity and high resolution MALDI-TOF-MS analysis, and (5) automated data processing (see Fig.1).

Fig.1 EPO purification using anti-EPO antibodies and glycosylation analysis. (Falck, 2017)

Anti-Glycan Antibody Services

The use of anti-glycan antibodies is capable of isolating glycans/glycoproteins that share common determinants. However, there is a limited source of anti-glycan antibodies that target different glycan families and epitopes. As an industry that has been concentrated on antibody development for years, Creative Biolabs now offers high-quality anti-glycan antibody development services to clients globally to boost their cutting-edge projects.

With highly-qualified and motivated staff as well as years of experience in the antibody development field, we will work closely with you step by step to satisfy every specific demand. If you are interested in our services, contact us to discuss your requirements.

Reference:

1. Falck, D.; et al. Affinity purification of erythropoietin from cell culture supernatant combined with MALDI-TOF-MS analysis of erythropoietin N-glycosylation. Scientific Reports. 2017, 7(1): 5324.