Cells need to be evaluated when analyzing the number and function of a particular cell population. Phenotypic changes in immune cells are a direct method for evaluating effectiveness. Cell surface markers refer to membrane proteins embedded in the lipid bilayer structure of the cell membrane, including membrane antigens, membrane receptors, and other molecules. Cell surface markers are not only the material basis for mutual recognition between immune cells but also between cells or between cells and the matrix. Including receptors, MHC molecules, coordinated stimulation molecules, etc. The CD molecule is one of the most commonly used cell surface markers, which refers to the application of monoclonal antibody identification methods to identify the same differentiated antigen recognized by monoclonal antibodies from different laboratories, including the specific morphology, specific function and developmental stage of the cell. Creative Biolabs provides clients with the identification of immune cell surface markers to distinguish different immune cells and accelerate their immunology research.
Fig.1 Some immune cells and their surface markers.¹
Creative Biolabs offers the following methods to help you identify the immune cell phenotype you need:
Fig.2 Cell marker expression and cell subtype.²
Fig.3 Proportion of advanced and stable cell subtypes.²
The above is a schematic diagram of the analysis of the immune composition of peripheral blood lymphocytes using mass cytometry analysis (cytometry by time-of-flight, CyTOF). Figure 2 is a heat map of a single-cell FlowSOM cluster, and the results are shown as common T cell clusters. Figure 3 is the relative expression of the markers detected by CyTOF.
Immune cells perform specific functions in the immune system. Immune cell markers can be used to identify and distinguish between different types of immune cells. Below we have listed common immune cells and their surface markers:
Cell Type | Surface Marker |
---|---|
B cells | CD1c, CD10/Neprilysin, CD19, CD21, CD22, CD27, CD40, CD95, IgM, IgD, BCMA |
Dendritic cells | CD11c, CLIP-170, Fascin, MADDAM, NLDC-145, MHCII (HLA-DR), CD33, CD123, CD16, CD128b |
Granulocytes |
|
Helper T Cells | BTLA, CD3, CD4, CD8, CXCL13, IFN-gamma, IL-4, IL-2, IL-5, IL-9, CCL17, CCL22, IL-21, IL-22 |
Innate Lymphoid Cells | CD3, CD4, CD45, CD49a, CD69, IFN-gamma, TNF-alpha, ICOS, IL-4, IL-5, IL-9, GM-CSF, NKG2A |
Macrophages | CD68, CD64, CSF-1R, F4-80 (mice), CD11b |
Monocytes | CD14, CD16, CD115, Ly-6C |
Myeloid-derived Suppressor Cells | CD11b/Integrin alpha M, CD14, CD15, CD11c |
Regulatory T Cell | CD3, CD4, CD5, CD14, CD19, Galectin-1, IL-10, IL-35, GITR, TGF-beta |
Creative Biolabs has always provided expertise and timely communication throughout the service.
If you are interested in our immune cell surface marker identification service, please feel free to contact us. We also offer more Bioassay services, if you are interested, please click to check it out!
References
For Research Use Only | Not For Clinical Use