B Cell based Flow Cytometry Assay Service
Creative Biolabs' B cell-based flow cytometry assay delivers comprehensive solutions and key problem-solving capabilities tailored to the unique needs of your research. Creative Biolabs provides precise characterization of B cell populations, enabling deep insights into immunological status, disease progression, and therapeutic response. Whether you need to assess vaccine efficacy, profile an autoimmune disease, or monitor CAR-T cell therapy, our assays provide the actionable data you need.
Introduction What We Can Offer Workflow Why Creative Biolabs Customer Reviews FAQs Related Services Contact Us
Application of Flow Cytometry in Antigen/Antibody Discovery
Accurate antigen epitope information is crucial for antibody discovery. However, isolating antigenic epitopes from thousands of potential epitope candidates is time-consuming. Flow cytometry has been optimized to a high-throughput, sensitive, and versatile technology for epitope binning by monitoring antibody-specific binding to antigen epitope. High-throughput flow cytometry is broadly used for analyzing multiple parameters for a large number of candidates using a single cell suspension or particles.
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Fig.1 Applications of Flow Cytometry. 1, 3
What We Can Offer
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Automated and High Throughput
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High Sensitivity
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Multiplex and High Efficiency
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Automated sample acquisition and sample preparation system.
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Screen more than 16000 samples in a week.
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Small sample volumes are needed.
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Sensitive, reliable, and reproducible data.
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Simultaneously analyze multiple antigen peptides in an assay.
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Flow Cytometry Assay Service at Creative Biolabs
Fig.2 Illustration of the yeast display technology. 2, 3
The following workflow is designed to provide a comprehensive and detailed approach to B cell analysis, ensuring accurate and meaningful results for your project. This systematic, multi-step process minimizes technical variability and maximizes data fidelity, ensuring the cellular profiles generated are robust and reliable for all downstream applications.
Fig.3 Workflow of the B cell-based flow cytometry assay process.
Highlights
Antigen-Specific B Cell Identification
We can precisely identify and characterize B cells that are specific to a particular antigen. This is a crucial application for understanding immune responses to vaccines and infectious diseases like SARS-CoV-2.
Intracellular and Functional Analysis
Our assays can be used to analyze not just surface markers, but also intracellular molecules like transcription factors and phosphoproteins. This provides deeper insights into the functional status and signaling pathways within B cells.
Tissue-Level Immunology
Our expertise extends to complex samples beyond blood. We have specialized protocols for isolating B cells from tissues and other biological fluids, providing a complete picture of the immunological landscape in different organs.
Advanced Data Interpretation
We don't just provide data; we provide insights. Our team leverages advanced computational analysis, including deep learning, to interpret high-dimensional datasets and identify meaningful trends that might be missed by conventional methods.
Discover how a partnership with us can streamline your research - Get a quote today.
Customer Reviews
Exceptional precision
Using Creative Biolabs' B cell-based flow cytometry assay in our research significantly improved the identification of rare B cell subsets. Their unmatched expertise in panel design and data analysis helped us overcome challenges with low cell counts and complex gating strategies. - J*s H**y.
Actionable insights
Creative Biolabs' service provided critical data on antigen-specific B cells, which was essential for our vaccine efficacy studies. Their team was incredibly helpful in addressing our specific concerns about sample viability and experimental controls. - M*y S***h.
FAQs
How does your assay differ from other methods like ELISA or ELISPOT?
Our flow cytometry assay provides a multi-parameter analysis at the single-cell level, allowing you to not only quantify B cells but also to determine their immunophenotype, activation status, and subset distribution simultaneously.
What is the minimum number of cells required for your assay?
The required cell number depends on the specific project and the rarity of the B cell population of interest. Our team can work with you to optimize the experimental design and ensure we can get the most out of your valuable samples.
Related Services
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We provide specialized services for analyzing immune cell populations within the tumor microenvironment to support immuno-oncology research and drug development.
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Chemokine and Cytokine Measurement
We provide comprehensive services for the measurement of chemokine and cytokine production to help you assess the immune response and inflammatory status of your samples.
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How to Contact Us
The flow cytometry assay is suitable for early characterization and identification of epitopes. This valuable assay enables you to advance the best epitope-based drug for future application when combining with other epitope analysis assays, such as functional assay and next-generation sequencing. If you are interested in our services, please contact us immediately.
References
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Robinson, J Paul et al. "Flow Cytometry: The Next Revolution." Cells vol. 12,14 1875. 17 Jul. 2023. https://doi.org/10.3390/cells12141875
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Uchański, Tomasz et al. "An improved yeast surface display platform for the screening of nanobody immune libraries." Scientific reports vol. 9,1 382. 23 Jan. 2019. https://doi.org/10.1038/s41598-018-37212-3
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Distributed under an Open Access license CC BY 4.0, without modification.
