Mixed Lymphocyte Reaction (MLR) based Dendritic Cell & T Cell Agonist Assay Service
Background Workflow Advanced Readouts Publication Why Choose Us FAQs Customer Review Related Services Contact Us
Mixed Lymphocyte Reaction (MLR) Insights: Advancing Immune Research and Therapies
MLR assays are integral in assessing the interaction between CD4+ T cells and antigenic peptides presented by class II major histocompatibility complex (MHC) molecules on antigen-presenting cells (APCs). Among APCs, dendritic cells (DCs) are the most potent, renowned for their ability to present antigens and activate T cells. In the tumor microenvironment, DCs play a pivotal role in inducing and maintaining anti-tumor immune responses, making them essential in cancer immunotherapy research.
Creative Biolabs provides comprehensive MLR assay services designed to evaluate the proliferative ability of lymphocytes under short-term in vitro culture conditions, following stimulation by foreign antigens. This assay is a crucial tool in immunology research, particularly for understanding T-cell responses, transplant compatibility, and the development of immunotherapies.
Our MLR assay services are designed to study various immune interactions, including the activation of both CD4+ and CD8+ T cells, which are crucial for orchestrating an effective immune response. By analyzing T cell proliferation and cytokine production, researchers gain insights into how the immune system recognizes and responds to malignant cells, which makes the assay valuable for developing novel cancer treatments and immunotherapies.
Procedure of DC and T Cell Agonist Assays
Advanced Readouts: [3H] Thymidine Incorporation and IFN-γ Production
-
[3H] Thymidine incorporation: Measures T cell proliferation by incorporating radioactive thymidine into the DNA of dividing cells, providing a precise quantitative assessment of lymphocyte proliferation and immune activation.
-
IFN-γ production: As a crucial pro-inflammatory cytokine, IFN-γ is a marker of T cell activation and immune response. Measuring IFN-γ levels helps assess the intensity of the immune response, offering valuable insights into therapeutic effectiveness.
Publication Sharing
The MLR assay is a critical tool for assessing the effects of various agents on T-cell proliferation. In this study, the MLR assay was employed to investigate the impact of SULF A, a TREM2-agonist, on T cell proliferation in an allogeneic setting. By measuring the proliferative response of T cells to SULF A, the assay provides insights into its potential immunoregulatory properties. The results demonstrate how SULF A influences T cell activation and proliferation, offering valuable information on its role in modulating immune responses. This analysis is crucial for evaluating the therapeutic potential of SULF A and contributes to the broader understanding of its effects on immune system regulation.
Fig.1 T cells proliferation induced by SULF A.1
Why Choose Us?
At Creative Biolabs, we combine over a decade of immunological expertise with cutting-edge technology to deliver precise, reliable, and fast MLR-based agonist assays. Our team of world-class immunologists works closely with you to design customized solutions, ensuring your drug development projects get the high-quality data they need. With rigorous quality control, comprehensive readouts, and a commitment to reproducibility, we provide actionable insights you can trust—helping you accelerate your research with confidence.
Expertise in DC utilization
Our deep understanding of DC biology ensures optimal assay design, particularly for cancer and immune response studies.
High sensitivity and specificity
Our assays are designed to provide accurate and reproducible results, with sensitive detection of T cell responses and cytokine production.
Comprehensive data analysis
We offer detailed analysis and interpretation of results, helping researchers translate data into actionable insights.
Customized assays
We tailor our agonist assays to meet specific research needs, whether for basic research or drug development.
Contact our expert team today to discuss your project.
Frequently Asked Questions
Q1: What types of compounds can be tested using the MLR-based dendritic cell and T cell agonist assay?
A1: Our assay is highly versatile and can be used to test a wide range of immunomodulatory compounds, including small molecules, biologics (e.g., antibodies, recombinant proteins), and even cell therapies. We can work with you to optimize the assay for your specific therapeutic candidate.
Q2: How sensitive is the MLR assay for detecting subtle T cell activation?
A2: The MLR is a highly sensitive assay for detecting T cell activation. By combining robust proliferation measurements with multi-parameter flow cytometry for activation markers and multiplex cytokine analysis, we can detect even subtle changes in T cell responses.
Q3: How does this MLR-based assay compare to other in vitro T cell activation assays?
A3: While other assays exist, our MLR-based assay stands out due to its physiological relevance, mimicking the natural interaction between dendritic cells and T cells. This provides a more predictive in vitro model compared to non-specific T cell activators, offering a robust platform for evaluating true T cell agonists.
Customer Review
-
Enhanced Lead Prioritization
Creative Biolabs' MLR-based dendritic cell and T cell agonist assays delivered clear, quantitative T cell activation data, helping us prioritize lead compounds effectively. Their detailed reports revealed key potency differences, streamlining our decision-making. - Jn D*e
-
Mechanism of Action Elucidation
Creative Biolabs' MLR-based assays delivered key insights into our immunomodulator's mechanism. Their detailed T cell activation and differentiation analysis proved critical for our submission, with outstanding support throughout the project. - Ax K**r
Related Services
To further support your immunomodulatory drug development, Creative Biolabs offers a suite of complementary services that can be integrated with our MLR-based dendritic cell and T cell agonist assay:
Cytokine Release Syndrome Evaluation
Recombinant CAR cells and other macromolecular therapeutics can cause cytokine release syndrome (CRS) due to the rapid release of cytokines. Creative Biolabs evaluates CRS based on a revised severity rating system to help predict new, effective biomarkers for its management.
Learn More →
ELISpot Assay Service
Creative Biolabs, with over 10 years of experience, provides ELISpot assays to detect cytokines and measure antigen-specific T cells. This highly sensitive functional assay is a good choice for research in AIDS, cancer, infectious diseases, and autoimmune diseases.
Learn More →
How to Contact Us
Unlock deeper insights into immune responses with our comprehensive MLR-based DC and T cell agonist assays. By analyzing DC-T cell interactions, we provide a complete picture of therapeutic efficacy to advance your research. Let's collaborate—contact us today for tailored solutions.
Reference
-
Barra, Giusi, et al. "The immunoregulatory effect of the TREM2-agonist Sulfavant A in human allogeneic mixed lymphocyte reaction." Frontiers in Immunology 14 (2023): 1050113. Distributed under Open Access license CC BY 4.0, without modification.
