ELISpot Assay Service

Creative Biolabs provides a definitive solution for quantifying immune cell responses with unmatched single-cell resolution. Our service is designed to deliver precise, actionable data on the frequency of cytokine-secreting cells, enabling you to evaluate the efficacy of vaccine candidates, monitor the potency of immunotherapies, and characterize immune profiles in infectious and autoimmune diseases. We offer a comprehensive solution that moves beyond simple spot counting to provide full data analysis and interpretation, ensuring you receive a complete understanding of your results.

Introduction What We Can Offer Workflow Why Creative Biolabs Customer Reviews FAQs Related Services Contact Us

Introduction of ELISPOT Assay Service

ELISPOT is a good choice to detect cytokines spontaneously, such as IFN-gamma, TNF-alpha, IL-2, and IL-4, which are produced by peripheral blood lymphocytes. To measure antigen-specific T cells, which typically occur at low frequency in vivo, ELISPOT techniques are also highly sensitive functional assays. Creative Biolabs offers ELISPOT assays using commercially available or custom assays to support our clients' R&D projects, and we provide validation services.

For further information regarding how our services can be instrumental to your research objectives, request a consultation.

Fig.1 Standardization of cellular assay using a multi-density reference plate layout. (OA Literature) Fig.1 Design and output of a PBMC density gradient reference plate of ELISPOT assay.¹

What We Can Offer

Types of ELISPOT Assays	Applications of ELISPOT Assays
T-Cell ELISPOT Assays We can provide valuable information about the number of cytokine-producing cells for both CD4+ T cells and CD8+ T cells, crucial for understanding cell-mediated immunity. B-Cell ELISPOT Assays For B-cells, we can determine the specific isotype of produced antibodies, providing insights into the humoral immune response. Cytokine Profiling Our scientists are experienced in performing ELISPOT analysis for a single cytokine or a combination of two or more cytokines, allowing for multiplexed analysis from a single sample.	Measurement of the immune response to infection by B-cell ELISPOT assays Qualification of antigen-specific antibody-secreting cells in human PBMCs Assessment of next-generation therapeutics ELISPOT analysis can be used in multiple research areas, including allergy, infectious diseases, cancer, vaccination studies, and autoimmunity.

Types of ELISPOT Assays

Applications of ELISPOT Assays

T-Cell ELISPOT Assays
We can provide valuable information about the number of cytokine-producing cells for both CD4+ T cells and CD8+ T cells, crucial for understanding cell-mediated immunity.
B-Cell ELISPOT Assays
For B-cells, we can determine the specific isotype of produced antibodies, providing insights into the humoral immune response.
Cytokine Profiling
Our scientists are experienced in performing ELISPOT analysis for a single cytokine or a combination of two or more cytokines, allowing for multiplexed analysis from a single sample.

Measurement of the immune response to infection by B-cell ELISPOT assays
Qualification of antigen-specific antibody-secreting cells in human PBMCs
Assessment of next-generation therapeutics
ELISPOT analysis can be used in multiple research areas, including allergy, infectious diseases, cancer, vaccination studies, and autoimmunity.

Let Our Experienced Team Help You with the ELISPOT Assay

Core steps of the ELISPOT assay process. (Creative Biolabs Original)

Highlights

High-Throughput & Flexibility

Creative Biolabs provides optimized, high-throughput assays that are designed to handle large sample volumes with ease. Our ELISPOT services are highly flexible, capable of being applied to a wide range of readout formats to meet the specific needs of your unique research goals.

Quantitative & Comprehensive

The key cellular functions of immune cells, including both adaptive and innate immune responses, can be measured quantitatively using our ELISPOT platform. This gives you a clear and precise understanding of the immune dynamics in your study.

Proven Standardization

Using our common standard operating procedures, the ELISPOT assay can be fully standardized, resulting in a seamless translation of the assay from the laboratory into a preclinical or clinical tool. This ensures reliability and consistency across all stages of your project.

Unrivaled Expertise

Creative Biolabs stands apart with over two decades of experience in the life science industry. Our published data confirms our ELISPOT counts are highly reproducible and statistically robust, providing a foundation of reliability for multi-site clinical trials and rigorous research.

Discover how a partnership with us can streamline your research - Get a quote today.

Customer Reviews

Multi-Analyte Profiling
The team at Creative Biolabs provided exceptional support. Their ELISPOT assay was crucial for our multi-analyte cytokine profiling, and their clear data reports made it easy to interpret our results and prepare for publication. - L*ra M.
High Reproducibility
We needed a reliable way to compare the immune response across different patient cohorts for our autoimmune disease study. The reproducibility of Creative Biolabs' ELISPOT assay gave us confidence in our data, and the high throughput was a major advantage. - M*k S.

FAQs

What is the minimum sample size required for your ELISPOT assay service?

The minimum sample size required depends on the specific project, but we can typically work with a small number of PBMCs. Please contact our technical support team to discuss the specifics of your study, and we will help you determine the optimal sample size.

How does the ELISPOT assay compare to a standard ELISA?

While both are ELISA-based, the ELISPOT assay is a functional test that measures the frequency of individual cytokine-secreting cells, while ELISA measures the total concentration of a cytokine in a bulk sample. ELISPOT offers significantly higher sensitivity, enabling the detection of rare cell populations that might be missed by ELISA.

Related Services

T Cell Activation Analysis

This service complements ELISPOT by assessing T-cell proliferation and the expression of key activation markers. It can be used to monitor the potency of immunotherapies and characterize immune profiles.

Learn More →

B Cell-based Flow Cytometry Assay

This service is ideal for high-throughput antigen/antibody discovery, particularly for cancer epitope analysis, using flow cytometry to efficiently screen and analyze large numbers of candidates.

Learn More →

How to Contact Us

ELISPOT assay represents one of the central methods in clinical diagnostic settings for the investigation and characterization of antigen-specific T cells, for example, in the context of tracking immune responses to vaccines. For more information and a detailed quote, please contact us.

Reference

Sundararaman, Srividya et al. "High Reproducibility of ELISPOT Counts from Nine Different Laboratories." Cells vol. 4,1 21-39. 9 Jan. 2015. Distributed under an Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/cells4010021

Download our brochure

Loading case studies...

Online Inquiry