The production of custom antibodies can be accomplished by using different antigens, such as protein, peptide, DNA, cells, etc. Among them, full-length and peptides are the most widely used traditional immunogens. In recent years, the use of synthetic peptide antigens as a means of producing customized antibodies has increased significantly.
Creative Biolabs provides comprehensive customized peptide synthesis services as a fully integrated component of Native™ customized antibody production services, adding quality and convenience to customized antibody development projects.
Peptide antigens are produced synthetically from carefully selected short amino acid sequences of target proteins. When target proteins are limited or unavailable, they are a popular solution because they are easy to synthesize and use with high purity. The peptide is covalently coupled to the carrier protein, and the resulting conjugate is used for immunization. In this case, the antibody produced is said to be sequence-specific. Such antibodies can cross-react with corresponding proteins and the epitopes of which can be easily mapped and identified. Using this method, antibodies can be generated against selected regions, such as highly conserved regions, active sites, extracellular or intracellular domains, or post-translational/chemically modified regions.
Fig.1 Immune stimulation and antibody generation by self-assembled peptide antigen.1
The choice of peptide immunogen depends on the intended use, i.e. the peptide antibody should recognize the putative antigen in denatured form (Western blotting) or natural form (immunoprecipitation or immunoassay). Especially if the target is a native protein, several questions need to be noted. The common feature of immunogenic peptides is that they preferentially represent sequential epitopes. For practical reasons, the selected amino acid sequence is usually determined by B-cell epitope examination or by processing physicochemical characteristics (e.g., accessibility, hydrophilicity, flexibility) and/or structural characteristics (e.g., loops, bends).
Creative Biolabs provides several unique strategies to increase the chance of producing anti-peptide antibodies that recognize natural proteins. Our peptide antigen synthesis platform has the following advantages:
If you are interested in our services, please feel free to contact us for more details.
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