Protein Antigen Production ONLINE INQUIRY

Most of the immunogens used for antibody production are proteins. These can be pure proteins, glycoproteins, or lipoproteins. Creative Biolabs has many years of experience in the development of customized antibodies for a variety of targets, which brings a unique perspective to the production of protein antigens.

Introduction of Protein Immunogen

Choosing the proper immunogen is one of the most critical steps in any customized antibody production project. In most cases, protein antigens are generally regarded as the gold standard when it comes to the production of high-quality antigens for antibody generation purposes. With the structural complexity and size, proteins themselves are strong immunogens. And the unique ability to trigger immune responses and recognize the native conformation makes these proteins excellent alternatives to other antigen substitutes. In addition, antibodies produced from protein antigens recognize multiple conformational epitopes within the target protein, enhancing the selectivity and specificity of the antibody.

Fig.1 Influence of the antigen nature on the corresponding antibody levels.¹

Protein Immunogen Preparation Services

The protein expression expert team of Creative Biolabs has more than ten years of protein expression experience and has generated a variety of protein expression systems, including bacterial expression, yeast expression, mammalian expression, etc. According to the needs of customers, we will analyze the properties of antigen molecules to design antigens with better immunogenicity and provide you with higher-quality antibodies. When designing protein immunogens, the options we consider are:

• Use in vivo natural proteins isolated from tissues (not feasible if the abundance of the target protein is very low or very similar to other protein family members).
• Use in vitro expressed recombinant proteins. If you need to avoid cross-reactions with other members of the protein family, you need to carefully select overexpressed fragments.

We choose optimal expression vectors and expression hosts with different fusion tag proteins, resistance, and action elements to truly realize in-depth private customization services for customers.

Fig.2 Schematic methods of protein antigen conjugated to carrier molecules as pathogens.²

Features

• Professional recombinant protein production and purification technology, with a variety of antigen expression platforms, and multiple protein purification platforms such as nickel column, conjugation receptor, conjugation antibody, protein A/G, size exclusion chromatography, and ion-exchange chromatography, etc.
• We have a variety of culture media, high-efficiency expression vectors and high-density cell culture systems to fully improve the success rate of expression and save the cost of customers.
• Leading technical personnel and laboratory equipment support high-throughput, large-scale and rapid production.

In the one-stop service of antibody production using B cell sorting technology, Creative Biolabs has accomplished many challenging antigen preparation projects. If you are interested in our services, please feel free to contact us for more details.

References

1. Slamanig, Stefan A., and Martijn A. Nolte. "The bone marrow as sanctuary for plasma cells and memory T-cells: implications for adaptive immunity and vaccinology." Cells 10.6 (2021): 1508. Distributed under Open Access license CC BY 4.0, without modification.
2. Lu, Lantian, et al. "Chemical conjugation strategies for the development of protein-based subunit nanovaccines." Vaccines 9.6 (2021): 563. Distributed under Open Access license CC BY 4.0. The image was modified by extracting and using only part of the original image.

Related Sections:

• B-Cell Sorting Platform
• Applications
• Species
• Peptide Antigen Production
• Hapten-Carrier Conjugation
• Carrier Protein Immunogens

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