In recent years, three-dimensional (3D) cell culture systems have gained increasing popularity as powerful tools for stem cell research. Traditional two-dimensional (2D) cultures have limitations in mimicking the native tissue microenvironment, hindering the full expression of stem cell potential. Embracing the benefits of 3D culture allows researchers to recreate more accurate physiological conditions, leading to enhanced cell differentiation, maturation, and tissue-specific functionality.
At Creative Biolabs, we present a comprehensive guide for culturing stem cells in a 3D environment in this protocol.
Select suitable low-attachment cell culture plates or synthetic scaffolds with pore sizes compatible with the stem cell type of interest. Pre-treat the scaffolds according to the manufacturer's instructions, ensuring the surfaces are sterile and free from contaminants.
Prepare the stem cell suspension in the desired culture medium at the appropriate cell density. Gently seed the stem cells onto the 3D scaffolds or plates, taking care to distribute them evenly. Place the seeded scaffolds or plates in the incubator and allow the cells to attach for a predetermined period.
Carefully add fresh culture medium to the 3D cultures, ensuring that the stem cells remain undisturbed during the process. Repeat the medium renewal every 2-3 days to maintain the optimal growth conditions for the stem cells.
At various time points during the culture period, assess cell viability using a cell viability assay kit. Utilize a fluorescence microscope to observe and capture images of the 3D cultures, documenting cell growth and behavior over time.
The 3D culture of stem cells offers an innovative approach to investigating cellular complexity and functionality. As a leading provider of stem cell research solutions, Creative Biolabs is committed to supporting scientists worldwide in their stem cell culture research and exploring the mysteries of stem cells.
Reference
For Research Use Only. Not For Clinical Use.
