Feeder-independent stem cell culture offers unparalleled opportunities for exploring the potential of stem cells in tissue engineering, disease modeling, and therapeutic applications. At Creative Biolabs, we take immense pride in presenting our cutting-edge feeder-independent stem cell culture protocol.
This comprehensive and meticulously designed protocol allows researchers to maintain and expand stem cell populations. And we are committed to enhancing experimental reproducibility and consistency and helping researchers unlock the true regenerative potential of stem cells.
Dilute the ECM protein to the desired concentration using sterile PBS. Add the diluted ECM solution to the culture vessels and incubate at 37°C for 1 hour to allow for proper coating. Remove any excess ECM solution and store the coated vessels at 4°C until further use.
Rinse the stem cell culture flask with sterile PBS to remove any residual medium. Detach the stem cells using a gentle trypsin-EDTA solution, and neutralize the trypsin with an appropriate medium containing serum or protein supplements. Count the cells using a hemocytometer or an automated cell counter to determine cell concentration. Seed the desired number of stem cells into the ECM-coated culture vessels with the appropriate culture medium.
Place the culture vessels in a humidified incubator set at 37°C and 5% CO2. Change the culture medium every 24 to 48 hours, depending on the specific requirements of the stem cell line and its growth rate. Monitor the stem cell culture regularly under a microscope to assess cell morphology, proliferation, and overall health. Subculture the stem cells when they reach approximately 70-80% confluency to maintain an optimal growth environment.
With our protocol, researchers can embrace the forefront of stem cell research, unearthing the untapped potential of regenerative medicine. You can feel free to contact us for any special customization requirements.
References
For Research Use Only. Not For Clinical Use.
