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Anti-Human HER2 (OTI5C4) h(CD28-CD3Z) CAR Lentivirus Packaging Kit (XS-0622-YF103)


All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

This kit provides a complete lentivirus packaging system to produce anti-HER2 (OTI5C4) h(4-1BB-CD3ζ) CAR lentivirus, which enables CAR-T cells generation to target Human HER2. It contains plasmids that express all the viral proteins necessary, and CAR detection reagent, providing a full set of reagents for CAR lentivirus packaging and CAR expression evaluation. The expressed anti-HER2 CAR lentiviruses are HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells. These viruses constitutively express the scFv portion of anti-HER2 (OTI5C4) linked to 2nd generation CAR containing CD28 and CD3ζ signaling domains. CAR detection reagent can be used to detect the positive expression rate of CAR-T cells after lentiviral transduction.

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Description

  • Target
  • HER2
  • Components
  • This kit contains a CAR lentiviral vector, packaging vectors, CAR detection reagent, and 293 cells.
    1. A CAR lentiviral vector
    • Anti-HER2 (OTI5C4) h(CD28-CD3ζ) CAR
    2. Three packaging vectors
    • LentiArt™ pHelp1
    • LentiArt™ pHelp2
    • LentiArt™ pHelp3
    3. CAR detection reagent
    4. 293 cells
  • Size
  • 10 reactions; 20 reactions; 50 reactions
  • Storage Conditions
  • For details of the storage requirements, please see the User Manual for instructions.
  • Applications
  • CAR lentivirus packaging; CAR lentivirus transfection; CAR-T cell development; CAR-T cell therapy.

CAR Vector

  • CAR Generation
  • 2nd-generation CAR
  • Vector Name
  • pCDCAR1
  • Vector Length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Receptor Construction
  • scFv-CD28-CD3ζ

Target

  • Gene Name
  • ERBB2
  • Full Name
  • erb-b2 receptor tyrosine kinase 2
  • scFv
  • OTI5C4
  • Host Species
  • Mouse
  • Target Species
  • Human
  • Target Background
  • This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
  • Synonyms
  • NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
  • Related Disease
  • Numerous cancers

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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