pCDCAR1 CEA h(ζOX)(CAR-MZ042)

The vector of anti-CEA chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target human CEA. The T cells are genetically modified through transduction with a retroviral vector expressing scFv of anti-CEA antibody linked to CD3ζ and OX40 signaling domains. And the vector product was designed for the treatment of Colon carcinoma.


Details


Target
CEA
Target Species
Human
Targeting Cell Type
T cell
Targeting Diseases
Colon carcinoma
Generation
Second
Vector Name
pCDCAR1
Vector Length
~8kb
Vector Type
Retroviral
Receptor Construction
scFv-CD3ζ-OX40
Discription of Signaling Cassetes
CD3ζ
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.
OX40
OX40, also known as CD134 or TNFRSF4 is a member of the TNFR-superfamily of receptors which is not constitutively expressed on resting naïve T cells, unlike CD28. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation. The interaction between OX40 and its binding partner, OX40L (CD252) plays an important role in antigen-specific T-cell expansion and survival. OX40 and OX40L also regulate cytokine production from T cells and modulate cytokine receptor signaling. OX40 cosignaling in CAR improve redirected T-cell effector functions and enhance anti-tumor activity.
Antibody Clone
BW431/26
Antibody Host
Human

Target


Gene Name
carcinoembryonic antigen related cell adhesion molecule 5
Official Symbol
CEACAM5
Gene ID
Synonyms
CEA; CD66e; CEACAM5; carcinoembryonic antigen related cell adhesion molecule 5

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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CarcinomaOthers

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CONTACT US

USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-871-5806
Fax: 1-631-207-8356
Email:

Europe
Ringstrasse 4, 64401 Gross-Bieberau, Germany
Tel: 44-207-097-1828

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