Enzyme-linked Immunosorbent Spot (ELISpot) Assay for Immune Monitoring

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Enzyme-linked Immunosorbent Spot (ELISpot) may not only provide a potency assay to assess immune response, but also biomarkers of therapeutic efficacy. With an experienced team of in-house ELISpot experts, Creative Biolabs employs immuno-enzymatic ELISpots assay and fluorescent ELISpot assay to develop highly customizable solutions for immune monitoring. Our team will work with our clients to develop a complete solution for their biomarker testing needs.

ELISpot Assay: a Potential Immunologic Biomarker Assay

With the advent of several immune-based therapies appearing to have beneficial clinical effects, especially in cancer patients, the ability to monitor the immune response stimulated by those therapies will be critical. Assays will have to be simple, inexpensive, and highly accurate to be able to be adapted to pivotal Phase III clinical trials. ELISpot assay is one of the most commonly used methods to measure antigen-specific T cells in both mice and humans. ELISpot assay can assess a large variety of immune effectors as well as the multiple functions of those effectors. Validation of ELISpot as a potential immunologic biomarker assay with prognostic significance would ensure ongoing adaptation of the assay as a primary mode for measuring human immunity in clinical trials.

Fig.1 Overview of ELISPOT assay. (Schultheis, 2016)

ELISpot Assay for Immune Monitoring at Creative Biolabs

Equipped with a team of seasoned scientists with facilities and processes designed specifically to meet very challenging requirements in biomarker development for immune monitoring, Creative Biolab offers high-quality immuno-enzymatic ELISpots and fluorescent ELISpot assay to monitor the immune response. Our scientists are versed in performing ELISpot assays for immune monitoring.

Common ELISpot Assays for Immune Monitoring

Interferon-gamma (IFN-γ) ELISpot assay

IFN-γ is an abundant cytokine produced by T helper type 1 (Th1) cells and is frequently used to track specific CD8⁺ responses. IFN-γ ELISpot assay has been used extensively for the screening of immune responses in the development of vaccines for the prevention and treatment of diverse diseases. Creative Biolab is pleased to share our knowledge in IFN-γ ELISpot assay to facilitate our clients' research and project development.

Granzyme B ELISpot assay

Secreted mainly from CD8⁺ cytotoxic lymphocytes and natural killer cells, granzyme B may induce the killing of virus-infected and tumor cells. Previous studies have indicated that granzyme B ELISpot has an excellent correlation with the ⁵¹Cr-release assay for measuring cytotoxic activity of effector cells. The detection of granzyme B-secreting cells by ELISpot is well established and the granzyme B ELISpot is a more direct measure of cytotoxic cell activity because granzyme B is one of the primary molecules in effector cell-mediated killing. Creative Biolab is confident in providing our clients with granzyme B ELISpot assay with the best service.

Transforming growth factor-beta 1 (TGF-β1) ELISpot assay

Functioning as both a suppressor and a promoter and also regulating infiltration of inflammatory/immune cells and cancer-associated fibroblasts in the tumor microenvironment, TGF-β plays an important role in tumor initiation and progression. It has been reported that there was a significant correlation between the frequency of TGF-β1-producing cells and total secreted TGF-β1 in patients infected with Mycobacterium avium and TGF-β1 ELISpot assays are 100-200-fold more sensitive than ELISA in the detection of the cytokines from whole-cell secretions. Creative Biolabs is dedicated to offering first-class TGF-β1 ELISpot assays to meet our clients' development goals in a time-saving manner.

Enzyme-linked Immunosorbent Spot (ELISpot) Assay for Immune Monitoring

Key Advantages of Our ELISpot Assay Including but Not Limited to

ELISpot is adaptable to multiple cell types (e.g., T cells and B cells) as well as to multiple secreted products (e.g., cytokines and granzyme B).
Measures cell function, which may be a predictor of outcome after immunotherapy.
The lower limit of detection than flow-based immunoassays
Detection of polyfunctional T cells.
Detection of a range of cytokines, such as IFN-γ, tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, and IL-17.
Quantitative
Simple, inexpensive, and highly accurate.

Equipped with a world-leading Immune Monitoring Platform and professional scientific staff, Creative Biolabs is always dedicated to assisting our clients with the most comprehensive ELISpot assays to monitor the immune response. For more information on ELISpot assays, please feel free to contact us and further discuss with our scientists.

Reference

Schultheis, K.; et al. Optimized interferon-gamma ELISpot assay to measure t cell responses in the guinea pig model after vaccination. MyJoVE Corporation. 2016.