Engineering Liver Cancer Cells for Self-destruction

Synthetic gene circuits are emerging as a versatile means to target cancer with enhanced specificity and efficiency. Creative Biolabs has developed a platform that focuses on generating gene circuits able to locally and accurately produce immunomodulators exclusively expressed in targeted cancer cells, turning cancer cells against themselves. The method can be widely used in a variety of cancers.

Engineering Liver Cancer Cells for Cancer Therapy

Synthetic gene circuits are aimed to selectively engineer and insert genes into targeted living cells to perform desirable, tunable, and controlled logical functions. The widening synthetic circuits are already being used in different cell types and animal models for safe operation of gene- and cell-based therapies. Engineering of mammalian cells normally starts with the design and construction of a functional genetic circuit, which is subsequently delivered to appropriate primary cells, an immortalized cell line, or stem cells. Based on a platform of synthetic gene circuits for cancer immunotherapy, Creative Biolabs provides engineering liver cancer cells to help our clients the development of safer immunotherapy, which may lead to innovative therapeutics for cancers.

Liver Cancer Facts

• Liver cancer is the sixth most common cancer and the second leading cause of death from cancer around the world.
• Liver cancer is the fifth and seventh most common cause of cancer death for men and women, respectively.
• Globally, hepatocellular carcinoma accounts for 80% of all cases of primary liver cancer, intrahepatic cholangiocarcinoma for 15% of all cases, and other subtypes for the remaining 5%.
• Approximately 56% of liver cancer is caused by hepatitis B and 20% by hepatitis C in the world.
• Liver cancer has a high mortality rate, with an overall 5-year survival rate of 18% in the US. The 5-year survival rate of localized disease is 31% and falls to 2% for metastatic HCC.
• The number of new cases and deaths from liver cancer could rise by >55% by 2040.

Fig.1 Global burden of primary liver cancer in 2020 and predictions to 2040. (Rumgay, et al., 2022)

Studies Performed with Engineered Liver Cancer Cells

For the treatment of hepatocellular carcinoma, researchers engineered a synthetic oncolytic adenovirus system using the tumor-specific promoter to control toggle switch expression and a specific miRNA signature to selectively control viral replication and immunomodulation (through co-expression of IL-2, GM-CSF, and anti-PD-1 scFvs).

Engineering Liver Cancer Cell Service

• Engineering Different Liver Cancer Cell Lines

Human tumor-derived cell lines have been widely used as models for studying cancer biology. Liver cancer cell lines are frequently used in vitro tools to test candidate cancer therapy as well as to elucidate mechanisms of liver carcinogenesis. The different liver cancer cell lines all varied in differentiation status and metastatic potential. Our professional team provides state-of-the-art services for engineering liver cancer cell lines, thus turning cancer cells against themselves. We are still trying to expand our cell line repository to better support your research.

• Engineering Liver Cancer Cells via Different Genetic Methods

A synthetic genetic circuit is usually delivered to targeted cells through several approaches, such as viral transduction (retrovirus, lentivirus, adenovirus, adeno-associated virus), or non-viral delivery systems. Targeted genome editing tools such as CRISPR/CAS9 system enabled more precise genetic manipulation to optimize cell engineering and function. Scientists at Creative Biolabs are confident to complete your cell engineering projects in far less time. The following are delivery strategies you can choose to achieve your goals.

• Several Strategies for Engineering Cancer Cells

Synthetic biology has become an innovative solution for the design of new, effective strategies in many fields including cell-based immunotherapies that involve various cytokines and their signaling pathways. Creative Biolabs provides several strategies to design and construct a functional genetic circuit for cancer immunotherapy.

Creative Biolabs provides a one-stop platform for the development of engineered liver cancer cells for self-destruction. Our systems offer a rapid and simple workflow for the whole project. In addition to cell line development, we also provide services to validate and assess cell performance in vitro and ex vivo.

Self-destruction Assays

Toxicity Assessment

Engineering Cancer Cells for Self-destruction

Ex Vivo Immune Reactivity Assessment

Assessment of the Circuit Performance

All projects are discussed in-depth with the client before the start of a project. We will evaluate the technical feasibility, cost of the project and time frame, etc. Please feel free to contact us to know more about our custom services.

References

1. Rumgay, H.; et al. Global burden of primary liver cancer in 2020 and predictions to 2040. Journal of Hepatology. 2022, 77(6): 1598-1606.
2. Huang, H.; et al. Oncolytic adenovirus programmed by synthetic gene circuit for cancer immunotherapy. Nature communications. 2019, 10(1): 1-15.

For Research Use Only | Not For Clinical Use