Creative Biolabs is a full-service antibody discovery company, focusing on the next generation of Native™ antibody discovery. Based on the solid technology platform, we are proud to provide antigen-specific B cell isolation services using antigen labeling multiparameter FACS for rapid Native™ antibody development.
Fluorescence-activated cell sorting (FACS), a specialized type of flow cytometry, is an essential immunological tool for rapid live cell sorting. It is a laser-based ultrahigh-throughput technique that is capable of screening, sorting, and identifying a defined cell subset from heterogeneous cells. When the fluorescence-labeled cell suspension flows through the channel of the flow cytometer, the fluorescent markers on the cell surface are excited or stimulated by a laser beam producing the scattered light signal and fluorescence signal. Usually, the cell flow is arranged to pass through the laser beam one cell at a time. By detecting the intensity of light signals, the biological characteristics and functional status of cells can be qualitatively or quantitatively detected.
FACS enables the isolation of the luminescent cell subpopulations according to the fluorescence intensity and wavelength of the emitted light, and the monoclonal cell sorting, cell identification, classification, quantification, and isolation, by which the sorting cell purity even reaches up to >95%. Since the invention of FACS in the late 1960s, FACS has been extensively used in immunology, oncology, pharmacology, molecular biology, and other disciplines.
Fig.1 FACS for positive cell selection.1
In order to successfully generate naturally paired monoclonal antibodies, it needs to obtain the gene information of the target antibody, which requires first isolating the specific antibody-secreting B cells or plasma cells from the immune individuals. Compared with B cell random isolation, antigen-specific B cell isolation for antibody development is much more efficient and labor-saving.
The conventional FACS technology is often applied for screening and isolating a defined cell subset, such as B lymphocyte subsets with identical surface markers, from samples using labeled antibodies. Based on FACS technology, antigen labeling multiparameter FACS has been developed for antigen-specific B cell isolation in high throughput and efficient manners. Different from FACS, antigen labeling multiparameter FACS requires not only fluorescent-labeled antibodies against B cell surface markers (recognizing B lymphocyte subset) but also fluorescent-labeled target antigens to recognize B cell surface membrane antibodies (confirming antigen-specific B cells). This antigen labeling multiparameter FACS is characterized by high throughput, high specificity, and high precision, which is the most advanced antigen-specific B cell sorting technology for Native™ antibody development. In addition, multiparameter FACS is capable of analyzing tens of thousands of cells at high speed, and can simultaneously measure multiple parameters from a single cell to obtain more cell information and select B cells at various stages for different studies.
Fig.2 FACS for the recombinant mAbs production.2
Creative Biolabs provides a comprehensive range of products and services for the rapid Native™ antibody discovery of various species, covering immunization, single B cell isolation, and antibody gene sequencing and cloning, to antibody generation. If you are interested or have any questions, please don’t hesitate to contact us.
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