Cynomolgus Monkey Heart (NHP-TI001)

The article focuses on the detailed study of the heart muscle in Cynomolgus monkeys, particularly emphasizing the role and diversity of tropomyosin (TPM) isoforms. Tropomyosin, a key protein in muscle contraction, exists in different forms (isoforms) that contribute to the heart's function. The research highlights the genetic similarity between Cynomolgus monkeys and humans, with about 90% DNA similarity, making these monkeys a valuable model for biomedical research, especially for studying human heart diseases.

Significant findings include the cloning and sequencing of various sarcomeric TPM isoforms from Cynomolgus monkey hearts and skeletal muscles. The study reveals a unique expression pattern of these isoforms in the monkey heart, with TPM1α being the dominant sarcomeric isoform present, unlike human hearts where other isoforms like TPM1κ, TPM2α, and TPM3α are also detected. This suggests notable differences in cardiac protein expression between humans and monkeys, potentially influencing heart function and disease.

Quantitative analyses provide insights into the expression levels of different TPM isoforms in Cynomolgus monkey heart and skeletal muscles, facilitating comparisons with human TPM expression. This research is crucial for understanding heart muscle function and its variations across species, offering a foundation for developing treatments for heart diseases.

Figure 1 Relative and absolute expression of TPM1& and TPM1' in the Cyn heart and skeletal muscle. (a) Relative expression of TPM1& using the dCt method. (b) Fold change of TPM1& using the ddCt method. (c) Relative expression of TPM1' using the dCt method. (d) Fold change of TPM1' using the ddCt method. (e) Estimation of the absolute copy number of TPM1&. (f) Estimation of the absolute copy number of TPM1'.

Figure 2 Relative and absolute expression of TPM2& in Cyn striated muscles. (a) Relative expression of TPM2& using the dCt method in the Cyn heart and skeletal muscle. (b) Fold change of TPM2& in the Cyn heart and skeletal muscle using the ddCt method. (c) Determination of the absolute copy number of TPM2a in the Cyn heart and skeletal muscle.

Figure 3 Relative and absolute expression of TPM3& in the Cyn heart and skeletal muscle. (a) Relative expression of TPM3& using the dCt method. (b) Fold change of TPM3& using the ddCt method. (c) Estimation of the absolute copy number of TPM3& in the Cyn heart and skeletal muscle.

Figure 4 Relative and absolute expression of TPM4& in Cyn striated muscles. (a) Relative expression of TPM4& using the dCt method in the Cyn heart and skeletal muscle. (b) Fold change of TPM4& in the Cyn heart and skeletal muscle using the ddCt method. (c) Determination of the absolute copy number of TPM4a in the Cyn heart and skeletal muscle.

Reference

1. Dipak K. Dube., et al. "Tropomyosin Isoform Diversity in the Cynomolgus Monkey Heart and Skeletal Muscles Compared to Human Tissues" Biochemistry Research International.(2023)