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Anti-Mesothelin (m912)-Megf10 CAR-MA (CARMA-W1180)


All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-Mesothelin chimeric antigen receptor (CAR) is constructed for the engineering of macrophage cells to target human Mesothelin and induce target cell phagocytosis. The macrophage cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-Mesothelin antibody (m912) linked to Megf10 signaling domains. The vector product was designed for the treatment of PDA (up to 100%), MPM (85%), Ovarian (70%), lung adenocarcinoma (53%, advanced; 69%, early stage), GBM.

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Details

  • Target
  • Mesothelin
  • Cell Type
  • Macrophage
  • Targeting Diseases
  • PDA (up to 100%), MPM (85%), Ovarian (70%), lung adenocarcinoma (53%, advanced; 69%, early stage), GBM
  • Vector Name
  • pCDCAR1
  • Vector length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Size
  • 10 µg
  • Storage
  • Store at -20°C for long term.

CAR Components

  • Promoter
  • EF1a
  • scFv
  • m912
  • Hinge
  • CD8
  • TM
  • CD8
  • ICD
  • Megf10
  • Marker
  • GFP or N/A
  • Receptor Construction
  • scFv-hinge(CD8)-TM(CD8)-ICD(Megf10)
  • Discription of Signaling Cassetes
  • Megf10:
    Megf10 is used as the CAR intracellular signaling domain. As a phagocytosis receptor, Megf10 has cytosolic Immunoreceptor Tyrosine-based Activation Motifs (ITAMs) that are phosphorylated by Src family kinases. Based on this mechanism, Megf10 signaling can induce macrophage phagocytosis which has been demonstrated in the published article.
    It is a membrane receptor involved in phagocytosis by macrophages and astrocytes of apoptotic cells. It's a receptor for C1q, an eat-me signal, that binds phosphatidylserine expressed on the surface of apoptotic cells

Target

  • Target
  • Mesothelin
  • Target Species
  • Human
  • Clone
  • m912
  • Host
  • Human
  • Gene Name
  • Mesothelin
  • Introduction
  • This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
  • Alternative Names
  • MPF; SMRP; MSLN; Mesothelin

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Customer Reviews and Q&As

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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