Immortalized Canine Cardiac Fibroblasts-SV40

Cat#: AHQ-1025-Q3

Immortalized Canine Cardiac Fibroblasts-SV40 were generated via lentiviral transfection of the SV40 large T antigen, conferring extended proliferation capacity and puromycin resistance. The cells maintain fibroblast morphology and are suitable for studies of cardiac remodeling, fibrosis, and myocardial physiology.

Inquiry Datasheet
Fig.1 Obesity can be counteracted by gene regulation.
Specifications Growth Information Shipping and Storage
Cell Type Cell Line
Source Heart
Species Canine
Cell State Immortalized
Genetic Characteristics Stably expresses SV40 large T antigen; puromycin resistant
Growth Characteristics Adherent
Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)
Physical State Frozen Vial
Recommended Culture Medium Creative Biolabs-Immortalized Canine Cardiac Fibroblast Culture Medium
CO₂ Concentration 5%
Incubation Temperature 37°C
Subculturing Ratio Recommended 1:2 at the first passage after recovery
Shipping and Storage Shipped on dry ice. Store in liquid nitrogen vapor phase upon receipt.

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