Rat Magnetic Luminex Mutiplex Cytokine/Chemokine Array Kit-79 Plex (CAR-K-LX-011)

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Product Name
Rat Magnetic Luminex Mutiplex Cytokine/Chemokine Array Kit-79 Plex

Cat. No.
CAR-K-LX-011

Product Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected rat cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and urine.

Components
Rectangular 4-Well Multi-dish.
4 Rat Cytokine Array nitrocellulose membranes spotted with 79 different antibodies to rat cytokines.
Array Buffer 4.
Array Buffer 6.
Chemi Reagent 1.
Chemi Reagent 2.
Detection Antibody Cocktail, Rat XL Cytokine Array.
Streptavidin-HRP.
Transparency Overlay Template.
Wash Buffer Concentrate (25X).

Assay Type
Multiplex

Species Reactivity
Rat

Sample Type (General)
Cell culture supernates, cell lysates, tissue lysates, serum, and urine.

Shipping Condition
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage Conditions
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Dtection Coverage
CCL2/JE/MCP-1, G-CSF, MAG/Siglec-4a, CCL3/CCL4/MIP-1α/β, GDF-15, MMP-2, CCL5/RANTES, GM-CSF, MMP-3, CCL11/Eotaxin, Hepassocin, MMP-9, CCL17/TARC, HGF, Neprilysin/CD10, CCL20/MIP-3α, ICAM-1/CD54, NOV/CCN3, CCL21/6Ckine, IFN-γ, NT-3, CCL22/MDC, IGF-I, NT-4, Clusterin, IGFBP-2, Osteopontin (OPN), CNTF, IGFBP-3, Osteoprotegerin/TNFRSF11B, CX3CL1/Fractalkine, IGFBP-5, PDGF-BB, CXCL2/GROβ/MIP-2/CINC-3, IGFBP-6, Pref-1/DLK1/FA1, CXCL7/Thymus Chemokine-1, IL-1α/IL-1F1, Prolactin, Cyr61/CCN1, IL-1β/IL-1F2, RAGE, Cystatin C, IL-1ra/IL-1F3, RBP4, DPPIV/CD26, IL-2, Resistin, EGF, IL-3, RGM-A, EG-VEGF, IL-4, SCF, Endostatin, IL-6, Serpin E1/PAI-1, Fetuin A, IL-13, TIM-1/KIM-1/HAVCR, FGF acidic, IL-17A, TNF-α, FGF-7, IL-22, TWEAK/TNFSF12, FGF-21, Jagged 1, VCAM-1/CD106, Fibulin 3, LIF, VEGF, Flt-3 Ligand, Lipocalin-2/NGAL, WISP-1/CCN

Detection method
Fluorescent

Product Features
Avaliability: able to detect 79 rat cytokines simultaneously.
No specialized equipment requirement.
Compatible with LI-COR and chemiluminescence detection.

Principle of the Assay
The Rat Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Rat Magnetic Luminex Mutiplex Cytokine/Chemokine Array Kit-79 Plex (CAR-K-LX-011)

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