Sequencing Service for Veterinary (Pet) Antibody

Are you encountering challenges in the development of novel veterinary antibody therapeutics, such as prolonged development timelines, hurdles in ensuring species-specific efficacy, or difficulties in producing stable, high-affinity antibodies? Creative Biolabs' sequencing service for veterinary antibody is your solution. We accelerate your journey from discovery to clinic by providing precise, comprehensive antibody sequencing. Our cutting-edge de novo sequencing technology and advanced bioinformatics platforms empower you to obtain high-quality, full-length antibody sequences, laying a critical foundation for successful downstream development, including caninization / felinization, affinity maturation, and scalable manufacturing of your innovative pet therapeutics.

Service Introduction

Creative Biolabs provide the definitive amino acid sequence of your monoclonal antibody, a crucial step for the development, patent protection, and production of next-generation veterinary biologics. Knowing the exact sequences of both heavy and light chains is essential for designing safer, more effective treatments for companion animals. This foundational knowledge fuels smarter therapeutic engineering and precision development. Our service delivers the complete, accurate sequence of your antibody, enabling you to secure your intellectual property, mitigate risks associated with cell line instability or loss, and facilitate critical downstream applications such as antibody engineering, caninization / felinization and recombinant production. With a precise blueprint of your molecule, you can confidently advance your project, ensuring reproducibility and paving the way for subsequent research and development process.

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Workflow

Our streamlined and transparent workflow is designed for efficiency and accuracy, ensuring you receive reliable data to propel your project forward.

Antibody Sequencing Service Workflow. (Creative Biolabs Original)Fig 1. Veterinary Antibody Sequencing Workflow. (Creative Biolabs Original)

Sample Preparation and QC Upon receipt, your sample undergoes rigorous quality control to assess purity and concentration, ensuring it meets the requirements for optimal sequencing outcomes.
Antibody Subunit Separation and Digestion We begin by carefully separating the antibody into its heavy and light chains. Each is then digested with a set of highly specific proteases to generate a rich pool of overlapping peptides, ensuring comprehensive coverage for accurate sequence analysis.
LC-MS/MS Analysis The resulting peptide fragments are separated using high-performance liquid chromatography (HPLC) and analyzed by high-resolution tandem mass spectrometry (MS/MS) to determine the amino acid sequence of each peptide.
De Novo Sequence Assembly Our proprietary bioinformatics algorithms assemble the overlapping peptide sequences to reconstruct the full-length amino acid sequence of both the heavy and light chains de novo, without reliance on a reference database.
Data Review and Reporting The final sequences are meticulously reviewed by our scientific team to ensure complete coverage and high confidence. A comprehensive report is then generated and delivered.
Upon project completion, you will receive a detailed report containing:
  • The full-length amino acid sequences of both the heavy and light chains.
  • A comprehensive peptide coverage map illustrating the sequenced fragments.
  • Detailed mass spectrometry data and a summary of the sequencing strategy.

Why Choose Us?

Antibody Sequencing. (Creative Biolabs Original)

  • Unrivaled Accuracy and Coverage
    We utilize a multi-enzyme digestion strategy coupled with advanced mass spectrometry to ensure 100% sequence coverage of both the variable and constant regions of your antibody. Our robust process delivers a single, accurate sequence you can trust.
  • Species-Specific Expertise
    Our team possesses extensive experience in sequencing antibodies from a wide range of species, including canine and feline. This expertise is critical for developing species-specific therapeutics that minimize immunogenicity and maximize efficacy in companion animals.
  • Proprietary Technology for Complex Molecules
    We leverage state-of-the-art de novo protein sequencing technology and sophisticated bioinformatics. This allows us to overcome challenges associated with novel isotypes or unexpected post-translational modifications, providing reliable results for even the most complex antibodies.

FAQs

1. Can you sequence antibodies directly from a hybridoma cell line?
Yes, we can. We offer a service that starts directly from hybridoma cells. This is an excellent way to secure the sequence of your valuable antibody
2. How does your sequencing technology differ from traditional methods?
Our service is based on de novo protein sequencing using mass spectrometry. This method directly analyzes the antibody protein itself, ensuring that the sequence we deliver is the one of the actual, functional antibody, including any post-translational modifications.
3. What if my antibody is of a non-standard isotype or from an uncommon species?
Our platform is species- and isotype-independent. We have successfully sequenced a wide variety of antibodies from numerous species. Our team has the expertise to tackle the unique challenges presented by novel antibody formats.

Related Services

To further support your veterinary antibody development pipeline, Creative Biolabs offers a suite of complementary services:

(Creative Biolabs Authorized)Antibody Physicochemical Characterization

Our physicochemical characterization evaluates key quality attributes like stability, solubility, and aggregation potential, helping you make informed decisions about developability and manufacturability early in the pipeline.

(Creative Biolabs Authorized)Antibody Affinity Measurement

With precise techniques like SPR, we determine how strongly your antibody binds its target antigen. These insights are critical for selecting and refining your top lead candidates.

Partner with Creative Biolabs

Creative Biolabs is committed to delivering the vital data and expert guidance you need to advance the next generation of transformative veterinary therapies. Our industry-leading antibody sequencing service is your first step toward securing, characterizing, and optimizing your most promising candidates with confidence.

Ready to push your veterinary therapeutic project forward? Our expert team is here to guide you from the very first step.

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