At Creative Biolabs, disease-specific pMHC Nanomedicine Development Service furnishes comprehensive solutions. These solutions are engineered to advance your autoimmune disease research and therapeutic development. We deliver customized pMHC nanomedicines.

Workflow

At Creative Biolabs, disease-specific pMHC nanomedicine development service adheres to a structured workflow. This workflow is designed to ensure efficient and successful project completion.

• Project Consultation and Design
  Our team collaborates closely with your research team. We do this to meticulously define project objectives, target autoantigens, and pertinent HLA alleles. We then design the pMHC nanomedicine construct. This design encompasses peptide selection, MHC molecule selection, and linker design. The aim is to optimize binding characteristics and overall efficacy.
• pMHC Complex Synthesis and Characterization
  We synthesize and purify the pMHC complexes. We employ advanced recombinant protein expression and refolding techniques in this process. We also conduct rigorous quality control assessments by SDS-PAGE, HPLC, and mass spectrometry.
• Nanoparticle Formulation
  After synthesis, the pMHC complexes are incorporated into nanoparticles using biocompatible materials. We carefully fine-tune factors like particle size, stability, and release kinetics to improve delivery in vivo and boost therapeutic performance.
• In Vitro Efficacy Testing
  Our evaluation of pMHC nanomedicine biological activity involves conducting multiple in vitro assays, such as T cell activation, alongside cytokine release and Treg induction tests. These assays help confirm their ability to effectively modulate T cell responses.
• In Vivo Validation
  We evaluate the therapeutic potential using appropriate autoimmune disease models.
• Data Analysis and Reporting
  We generate detailed reports with comprehensive data analysis, including statistical analysis and visualization.

• Required Starting Materials: To initiate a project, clients typically furnish us with:
  • Target autoantigen sequence information (peptide sequence).
  • HLA allele information relevant to the target patient population.
  • Specific disease model or clearly defined research goals.

Technology

• Recombinant protein expression systems: We utilize a diverse portfolio of recombinant protein expression systems, including bacterial, yeast, mammalian cells, and cell-free systems. Each system provides distinct advantages.
• Peptide synthesis: We employ state-of-the-art peptide synthesis techniques for pMHC complex assembly.
• Nanoparticle formulation techniques: We utilize a diverse range of nanoparticle formulation techniques, which encompass self-assembly processes, emulsification methods, and microfluidic approaches.
• In Vitro and In Vivo Immunological Assays: We employ a comprehensive suite of in vitro and in vivo immunological assays, including T cell activation assays, cytokine release assays, Treg induction assays, and binding assays. These assays provide valuable insights. They allow us to accurately assess the capacity of pMHC nanomedicines to modulate T cell responses.

Why Choose Us?

• Expertise: Our team is comprised of highly experienced scientists. These scientists possess deep and extensive knowledge.
• Customization: We meticulously tailor our services. We do this to align precisely with your specific research needs. This ensures the attainment of optimal and highly relevant results.
• Technology: We employ a suite of advanced technologies, which can be applied in pMHC complex synthesis, nanoparticle formulation, and in vivo validation.
• Quality: We adhere to stringent quality control standards to ensure the delivery of consistently high-quality products and reliable data.

What We Can Offer

• Custom pMHC nanomedicine design and synthesis
  We provide end-to-end solutions. These solutions cover the design and synthesis of custom pMHC nanomedicines.
  • Target identification and validation: We provide assistance in the identification of relevant autoantigens. We also help in the identification of HLA alleles. This ensures that they are appropriate for your specific target disease and the relevant patient population.
  • Peptide design and optimization: We employ advanced computational tools. We also utilize a range of sophisticated experimental techniques. These are deployed in the design and optimization of antigenic peptides. The goal is to achieve enhanced binding affinity and improved stability.
  • MHC molecule selection and production: We produce high-quality MHC molecules. We utilize recombinant expression systems in this production process. This ensures proper protein folding and optimal functionality.
  • pMHC complex assembly and purification: We assemble and purify pMHC complexes. We adhere to optimized protocols.
• In vitro and in vivo efficacy testing
  We offer a wide range of in vitro and in vivo assays to rigorously evaluate the efficacy of your pMHC nanomedicines.
  • T cell activation assays: This measurement is performed using flow cytometry and ELISA in response to pMHC nanomedicines.
  • Cytokine release assays: We quantify the production of both pro-inflammatory and anti-inflammatory cytokines, which enables us to accurately assess the immunomodulatory effects of pMHC nanomedicines.
  • Treg induction assays: We evaluate the capacity of pMHC nanomedicines to determine their ability to induce the differentiation and expansion of regulatory T cells (Tregs).
  • Binding assays: We measure the binding affinity between pMHC nanomedicines and T cell receptors (TCRs) by surface plasmon resonance (SPR) and biolayer interferometry (BLI).
• Pharmacokinetic and pharmacodynamic studies: We conduct pharmacokinetic (PK) and pharmacodynamic (PD) studies to rigorously evaluate the in vivo behavior of pMHC nanomedicines.
• Consultation and technical support: Our team of highly skilled experts provides comprehensive consultation and technical support. We do this to design and execute your studies effectively. We also aid in the interpretation of the resultant data. Furthermore, we offer guidance and support in optimizing your pMHC nanomedicine development strategy.

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Extended Services

Creative Biolabs offers a range of complementary services. These services provide support for your autoimmune disease research. These include:

• Custom peptide synthesis: We offer custom peptide synthesis services, which find in a wide array of applications, including:
  • Epitope mapping
  • T cell assays
  • Drug discovery initiatives
  • Biomarker identification
• Recombinant protein expression: We provide high-quality recombinant protein expression services and the following related service.
  • Protein engineering
  • Cell line development
  • Scale-up production
  • Protein purification
• Antibody development: We offer comprehensive antibody development services. Our services cover the entire antibody development process. This spans from the initial crucial steps of antigen design and synthesis to the final stages of antibody purification and thorough characterization.
• Cell-based assays: We develop and perform cell-based assays. These assays play a crucial role. They allow for a meticulous evaluation of the efficacy and safety of pMHC nanomedicines. Our cell-based assays, for example, include:
  • T cell activation assays
  • Cytokine release assays
  • Treg induction assays
  • Cell proliferation assays
  • Apoptosis assays

These assays furnish valuable information. This information pertains to the mechanism of action of pMHC nanomedicines.

Contact Our Team for More Information and to Discuss Your Project

For Research Use Only | Not For Clinical Use