Single Domain Antibody (SdAb) Discovery Service for Immunoassay and Biosensor Affinity Probe
Recently, single domain antibodies (sdAbs) have been used as affinity probes in immunoassays and biosensors for the diagnostics of serious human diseases and the quantitative analysis of contaminants and toxins in food and environmental samples. With the help of our highly experienced staff, Creative Biolabs provides novel sdAb discovery and development services for our global customers to generate high-quality affinity probes to meet the immunoassay and biosensor development requirements.
The development of biosensors is a significant area of research due to the necessity for sensitive analyte detection devices in various critical fields, including medical diagnostics, environmental monitoring, and food safety analysis. As a superior solution, single domain antibodies are being designed into cost-effective, rapid immunoassays for screening and testing both humans and animals in remote locations or field settings without the need for a cold chain. Additionally, sdAbs are utilized as capturing and detecting agents in immediate electrochemical tests to achieve higher diagnostic sensitivity. These sdAbs can also function as capture agents in other systems, such as enzyme immunoassays (EIA) or surface plasmon resonance (SPR) assays, enabling early and highly accurate diagnosis of viral, bacterial, or fungal infections.
Advantages of sdAb for In Vitro Diagnosis
Many innovative biosensors rely on small, robust, highly specific affinity probes, where size and directional immobilization matter. Detection probes should ideally possess the following attributes: high accessibility, stability, and selectivity towards the target antigen, even in complex samples, along with cost-effective scalability for large-scale production. Because of the generally high specificity, high stability, low production cost in microbial systems, and easy tailoring, sdAbs have been viewed as potentially superior alternatives to conventional antibodies for in vitro diagnostics.
-
Their small size and lower aggregation propensity enable immobilization of sdAb molecules at high probe density on the surface of the well of microtitre plate or biosensor transducer, which leads to a substantial increase in the sensitivity of the immunodetection system. Moreover, sdAbs directionally immobilized onto solid sensor surfaces allow immunoreactions to occur very close to the sensor-solution interface, resulting in increased sensitivity for detecting antigens in complex biological samples.
-
The intrinsic stability and refolding competence of sdAbs allow the resistance to stringent washing and denaturing conditions used during harsh probe regeneration. The ability of sdAbs to retain their functionality under extreme chemical conditions is essential for detecting food contaminants extracted in high concentrations of protein-denaturing methanol.
-
sdAbs are particularly suited for controlled and reproducible coupling for in vitro detection because site-specific functional groups are quickly introduced, allowing covalent and oriented binding with minimal specificity and affinity loss.
In Vitro Diagnosis in Infectious Diseases
Early diagnosis using sdAb as an affinity probe is favorable, especially for highly pathogenic and potentially lethal pathogens or pathogens for which no specific cure exists. Obviously, early diagnosis of an infection or disease is essential for a speedy recovery. A wide range of sdAb-based in vitro diagnostic tests has been developed. For example, sdAbs have been effectively utilized to differentiate between Brucella and Yersinia infections in livestock. Diagnostic sdAbs for Trypanosoma parasites have also been developed without the requirement of purified antigens. Recently, a sdAb-based agglutination reagent has undergone evaluation for its efficacy in HIV diagnosis. sdAbs also have been used to monitor the presence of roundworm parasite (Toxocara) excretory-secretory proteins in human sera. These factors collectively indicate that employing single domain antibodies (sdAbs) to develop sensitive and selective biosensors for in vitro disease diagnosis is highly viable.
Creative Biolabs is a dedicated provider that strives to provide the highest quality sdAb development services. Please feel free to contact us to know how we can help you develop sdAbs for your projects.
We are offering highly customized CRO services to assist your Single Domain Antibody (sdAb) related projects. Please Contact Us for more details.