With years of experience, Creative Biolabs has developed a series of innovative stem cell-based platforms to provide chondrogenesis differentiation of mesenchymal stem cells (MSCs) service for our global customers. Our full-service packages are designed to meet the clients' project and specific requirements for MSC differentiation.
In the adult human, MSCs in bone marrow retain the capacity to proliferate and differentiate along with multiple cartilages. Recently, MSCs are interested as a promising cell source for in vitro chondrogenesis due to their ease of isolation and high expansion capacity. MSCs can be isolated from many vascularized tissues as they reside within the joint that may be considered a potential reservoir of chondroprogenitor cells. Common in vitro protocols for chondrogenic differentiation of MSC successfully induce expression of multiple cartilage-specific molecules, including collagen type II and aggrecan, and result in a chondrocyte-like phenotype.
The development of cartilage is initiated by a phase of condensation of mesenchymal precursor cells during embryogenesis. The cell-cell contact arising from condensation involving N-cadherin appears to be crucial for the onset of chondrogenesis. Therefore, functional N-cadherin is necessary for chondrogenesis of mesenchymal cells both in vitro and in vivo. Moreover, high cell density and cell-cell contact could facilitate chondrogenic induction of MSCs compared with monolayer culture. MSCs have an intrinsic differentiation program of endochondral bone formation induced by exposure to these specific reagents.
Fig.1 Chondrogenic lineage progression of MSCs. (Somoza, 2014)
MSCs can fulfill the requirements demanded of cells for tissue engineering of cartilage, as they can be conveniently manipulated in vitro to differentiate to chondrocytes for these purposes. In addition, MSCs can faithfully reproduce a chondrocyte phenotype, including hypertrophic stages. The potential of chondrogenic differentiation from MSCs is shown in many matrix-free and matrix-based cell culture systems. Now, the induction of in vitro chondrogenesis from MSCs displays well-established standard procedures. As a global leader of stem cell-based custom services, Creative Biolabs focuses on a powerful model system containing a condensed cell culture system and uses inducers to efficiently promote the chondrogenesis of MSCs to meet our customers' specific requirements.
Based on our deep understanding of various strategies, our scientists have built up one-stop solutions and considered different factors for the chondrogenic differentiation of MSCs, including but not limited to:
With professional scientists devoted to MSC differentiation, Creative Biolabs is dedicated to providing the first-in-class chondrogenesis differentiation of MSC service for our customers worldwide. Our services will greatly contribute to the success of your MSC programs. Please feel free to contact us for more information.
Reference
For Research Use Only. Not For Clinical Use.
