Mesenchymal stem cells (MSCs) are undifferentiated multipotent cells that possess great potential in tissue regeneration and cell-based therapy due to their multipotency. Application of MSCs requires their isolation and direction of their differentiation into the appropriate lineage. Creative Biolabs offers customized services for reliable in vitro induction of MSCs into osteogenic, adipogenic, muscular, and chondrogenic lineages.
MSCs have the ability to differentiate into a large number of specialized cell types following specific signals (e.g., Wnt and redox signals, microRNAs, reactive oxygen species). These include mesodermal (osteoblasts, chondrocytes, adipocytes, myocytes, cardiomyocytes), ectodermal (neuronal cells) and endodermal (hepatic, pancreatic, respiratory epithelium) lineages. Moreover, a fully differentiated cell from one lineage can switch into another mature cell type of MSCs, which is a phenomenon called genetic reprogramming or transdifferentiation. As a result, MSCs derived from different tissues can be readily isolated and expanded for both basic and biomedical research. It has to be noted that MSCs from different tissues have exhibited differences in their differentiation capacity and regeneration potential. Besides, increased age of donor and passaging of cultured MSCs also decrease differentiation potential.
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Fig.1 Differential potential of MSCs.
The processes by which MSCs commit to the adipose lineage and differentiate into adipocytes is termed adipogenesis. This process is triggered by signaling factors that induce conversion of MSCs to preadipocytes that differentiate into adipocytes. Thereafter, exposure of preadipocytes to differentiation inducers, such as insulin-like growth factor1, glucocorticoid, and cyclic AMP, triggers the expression of adipocyte genes and the production of adipocyte phenotype. With the use of appropriate cultures, Creative Biolabs can help induce the differentiation of your MSCs into both white and brown adipocytes.
Manipulations of MSCs toward osteogenic lineage differentiation to form bone is a focused area in recent years. To help facilitate these research areas, Creative Biolabs focuses on providing MSCs osteogenesis differentiation system for in vitro studies. Optimal culture conditions, including many selective, temporally specific growth factors (BMP-2 and VEGF), are allowed in our differentiation protocols to enhance osteogenesis differentiation efficiently.
The capabilities of MSCs to differentiate into chondrogenic lineages make it a promising approach for cartilage repair and regeneration. The chondrogenic differentiation of MSCs is regulated by many factors and thus it relies on exposure to specific reagents (mainly inductive morphogens) to push MSCs to develop into specific phenotypes. Creative Biolabs has rich experience in promoting the chondrogenic differentiation of MSCs and enhancing the chondrogenic potential of dedifferentiated chondrocytes.
Creative Biolabs conducts custom-specific contract research based on years of experience gained in a number of successful development projects. We can provide both individual service modules and full packages of services. Our state-of-the-art technologies and highly-qualified personnel guarantee that we can deliver maximum quality and flexibility in all phases of the product lifecycle. Please feel free to contact us for more information.
For Research Use Only. Not For Clinical Use.
