Osteogenesis Differentiation of MSC Service

The stem cell team at Creative Biolabs offers world-class expertise in the generation and differentiation of mesenchymal stem cells (MSCs) and uses this to provide solutions for your MSC needs. Our full-service packages are designed to meet the clients' project and specific requirements for osteogenesis differentiation of MSC.

MSC Osteogenic Differentiation in Health

MSCs are a population of stromal cells present in the bone marrow and most connective tissues, capable of differentiating into mesenchymal tissues such as bone and cartilage. MSCs from different sources are not necessarily equivalent. Indeed, they have shown significant functional differences and most certainly require specific optimal culture conditions and differentiation protocols for osteogenesis differentiation. MSCs can be used for differentiation into osteogenic lineages to form bone. The process involves three distinct stages from the commitment of osteoprogenitor cells, pre-osteoblasts differentiation, and mature osteoblasts formation.

Fig.1 Osteogenic differentiation of MSCs. (Infante, 2018)

• Regulation of Osteogenic Differentiation
• Runt-related transcription factor 2 (Runx2) and the transcription factor osterix (Osx) can initiate and continue osteogenesis.
• Bone morphogenetic protein 2 (BMP2) and FBJ murine osteosarcoma viral oncogene homolog B (FosB) increase osteogenesis and inhibit adipogenesis.
• Glycerophosphate and dexamethasone are used for the achievement of osteogenic differentiation in vitro.
• Increased expression of alkaline phosphatase (ALP) and osteocalcin (OC) serve as markers of osteogenesis.
• Some transcription factors, including CCAAT enhancer-binding protein beta (CEBPβ), gamma (CEBPγ), alfa (CEBPα), peroxisome proliferator-activated receptor-gamma (PPARγ), and Sp7 transcription factor (SP7) used for the control of the commitment of MSC differentiation to osteogenesis.

Service at Creative Biolabs

Creative Biolabs focuses on providing MSCs osteogenesis differentiation system for in vitro studies. Seasoned scientists at Creative Biolabs have set up a series of one-stop procedures to assist your cell model development with the highest efficiency and the best quality. We have established a cell culture system of MSCs that allows for osteogenic and adipogenic differentiation and trans-differentiation between both cell lineages. Also, selective, temporally specific addition of growth factors, such as BMP-2 and VEGF, appears to be an important strategy to enhance osteogenesis. We look forward to working with you in the future.

Features of Our Service

• A dedicated team of technicians and scientists
• An individually tailored service
• Access to reports and procedures
• Fast turnaround time
• Competitive pricing

Aided by our well-established platforms and experienced scientists, Creative Biolabs can provide osteogenesis differentiation service of MSC for our global customers. We prefer to selecting the best strategies according to your specific situation. Please feel free to contact us to learn more information or a detailed quote.

Reference

1. Infante, A.; Rodríguez, C. I. Osteogenesis and aging: lessons from mesenchymal stem cells. Stem cell research & therapy. 2018, 9(1), 1-7.

For Research Use Only. Not For Clinical Use.