Creative Biolabs is a leading service provider that focuses on iPSC generation. With years of experience, now we can provide the iPSC maintenance service for our customers all over the world.
Due to the similar properties compared with embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs) enable the next generation of cell therapies in clinical applications. Since iPSCs were generated from mouse embryonic fibroblasts and adult human somatic cells by introducing four defined reprogramming factors in 2006, several reprogramming strategies have been discovered for the iPSC generation. However, there are still several technical hurdles before a wide range of clinical implementation, such as the scalable, along with reproducible production of iPSCs. With years of experiment, scientists at Creative Biolabs have built two systems for iPSCs culture, which are feeder-dependent culture system and feeder-free culture system.
Fig.1 Flow chart for general maintenance and passaging of iPS cells grown on MEFs.
iPSCs are derived from adult cell types such as fibroblasts using various strategies. The common feeder-dependent culture system provided by Creative Biolabs is based on iPSCs maintained on a layer of inactive murine embryonic fibroblast (MEF) feeder cells. Under these conditions, the human iPSCs can be maintained for several passages without compromising cells' proliferation or pluripotency and differentiation potential. This feeder-dependent culture system can benefit from its high efficiency in a short time but sometimes limited by the requirement of significant time and effort.
In order to discover a novel culture system applicable for human iPSCs, both feeder-free and xeno-free conditions must be met. There are various matrices that can be used to replace feeder cells, such as CELLstart, synthetic polymers, matrigel, and recombinant proteins. Meanwhile, the available xeno-free media includes TeSR2, NutriStem, and Essential E8 medium. The next step is to identify an efficacious combination of matrix and medium, since medium require configuration to satisfy cells in new condition with potential problems such as detachment, poor metabolism, difficulty in proliferation and possible apoptosis. After a series of experiment, we have successfully developed the novel feeder-free culture system for human iPSCs using StemFit™ and rLN511E8. With the advantages of simple use, high expand, and reproduction, the human iPSCs with equivalent growth and differentiation potential can be generated from various tissues under our feeder-free and xeno-free conditions.
Fig.2 Feeder-free culture method for human iPSCs.
With our well-established iPSC generation technology, Creative Biolabs now provides different culture systems for iPSC generation. Based on your specific situation, we can also customize protocol to meet our clients' demands precisely. Creative Biolabs provides other services regarding iPSC generation and iPSC applications, please do not hesitate to contact us if you are interested in them.
References
For Research Use Only. Not For Clinical Use.
