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iPSC Culture Services


Nowadays, human induced pluripotent stem cells (iPSC) offer great resources for application in pharmaceutical development and regenerative medicine. With years of exploration in iPSC development, Creative Biolabs is dedicated to providing several iPSC culture services, including maintaince of iPSC, 3D culture of iPSC, as well as scale up culture of iPSC.

As we all know, the growth of any type of mammalian cell in vitro needs extracellular matrices, growth media, and environmental factors, as well as iPSC. However, the iPSC culture has several specific factors need took into consideration, which may influence the quality, robustness, and utility of a variety of iPSC culture methods.

Growth Medium Development for iPSC Culture

Growth medium plays the most important part in hPSC culture, and goes through a dynamic evolution since it was first used for hESC culture. The final goal for therapeutic application is to design a serum-free, xeno-free, and chemically defined medium, available for supporting the growth of almost all types of iPSC lines. Recently, researchers have developed chemically defined E8 medium, a derivative of TeSR1 containing 8 components. This novel medium is short of both serum albumin and β-mercaptoethanol. This E8 medium, combined with EDTA passaging, is a promising culture for a great number of iPSC and hESC lines, it also may promote episomal vector-based reprogramming efficiencies and experimental consistency.

Generation of different organoids using iPSCs in 3D environments. (Li, Y., 2017) Fig 1. Generation of different organoids using iPSCs in 3D environments. (Li, Y., 2017)

Extracellular Components

Extracellular components comprise a variety of organic matrices from animal cells, individual matrix proteins, hydrogel, synthetic surfaces, as well as some commercially well-defined and xenogeneic-free components.

Environmental cues

To date, environmental cues also serve an essential role in the hPSC culture, comprising cues from physical and physiological environments which promote hPSC growth, for example, humidity, acidity, temperature, osmosity, cell density, gas diffusion exchange, and modes of multicellular associations. Among these factors, consumption of oxygen and the modes of multicellular associations are the two most important.

We offer standard and customized iPSC culture services including mycoplasma testing and FBS lot evaluation. We are experienced with almost all types of culture formats such as traditional plates, flasks, roller bottles, and spinner vessels. Following are some leading services available in Creative Biolabs. We offer customized services to meet every customer’s need.

With professional scientists devoted themselves in iPSC culture, Creative Biolabs is dedicated to providing the first class iPSC culture services for our customers. Our services will contribute greatly to the success of your iPSC programs. Please contact us for more information and a detailed quote.

References

  1. Chen, K. G., (2014). “Human pluripotent stem cell culture: considerations for maintenance, expansion, and therapeutics.” Cell stem cell, 14(1), 13-26.
  2. Li, Y., (2017). “Engineering-derived approaches for iPSC preparation, expansion, differentiation and applications.” Biofabrication, 9(3), 032001.

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