In terms of the extensive experience in iPSC research, Creative Biolabs is the world-leading provider for iPSC generation and applications. Through years of effort, now we can provide a novel 3D culture system for the iPSCs expansion and differentiation.
With the capacities for indefinite expansion in vitro and differentiation into different cell types, induced pluripotent stem cells (iPSCs) show great potential for biomedical applications, which include high-throughput pharmacology, replacement therapies, toxicology screening, and tissue engineering. However, a technical problem that must be solved is the high demand for high-quality iPSCs. In general, iPSCs sense the key biological signals from the substrate to improve their survival and rapid proliferation. The conventional 2D culture system cannot achieve mass production of high-quality iPSCs because of the inherent heterogeneity, limited scalability and lack of repeatability. In order to break the bottleneck of iPSCs culture from 2D to 3D, a lot of efforts have been done, such as iPSCs in alginate microencapsulates, iPSCs on microcarriers, and iPSCs aggregates. But they are not able to solve the problem perfectly because of multiple restrictions.
Fig.1 Flow chart for the iPSCs generation.
In order to conquer these challenges, now Creative Biolabs has built a 3D culture system for the iPSCs expansion and differentiation based on a thermoreversible hydrogel. This material is a polymeric liquid at low temperatures and becomes solid when heated. In this case, the iPSCs can be mixed with the liquid at low temperature and then suspended and grown in a solid gel at 37 degrees. Finally, iPSCs can be harvested and passaged by reliquifying the gel at low temperature. In summary, our 3D culture system enables a long-term and serial expansion of multiple human iPSCs lines via a mild process (simply temperature changes from 4 degrees to 37 degrees). With these wonderful advantages, our 3D culture system might be useful at numerous scales, from basic biological investigation to clinical trials.
Fig.2 The cells in 2D and 3D culture system.
Based on our extensive experience and well-recognized platform, Creative Biolabs is confident in offering the best services and high-quality products to meet the special needs of our customers all over the world. Apart from the 3D culture systems, we can also provide various other services regarding iPSCs generation and iPSCs applications. If you are interested in our services, please feel free to contact us for more details.
References
For Research Use Only. Not For Clinical Use.
