Chromatin Accessibility Sequencing Service
With world renowned expertise in cancer epigenomic researches, Creative Biolabs developed advanced epigenomic sequencing platforms to deep your understanding in oncology. Our scientists operates in state-of-the-art facilities to provide superior quality data to support your epigenomic studies.
Background
The whole genome is efficiently packaged as nucleosomes and high-order chromatin architecture. This structure prevents the binding of transcription factors, and other DNA-binding factors, leading to gene silencing. Transcription activation is highly associated with disruption of nucleosome organization through cis-acting DNA elements including enhancer, promoters, insulators and other chromatin-binding factors. Chromatin accessibility across the genome is actively and dynamically remodeled (see Fig.1) to regulate the gene expression and cell programming which is regulated by various cis-acting DNA elements. Therefore, studying the chromatin accessibility reveals the epigenomic mechanisms involved in specific cellular processes in healthy and diseased states.
Our Chromatin Accessibility Sequencing Service
Empowered by our advanced and innovative epigenomic platforms, our scientists developed chromatin accessibility sequencing services to measure the chromatin accessibility, explore the mechanism of transcription factors in accessibility remodeling, illustrate the role of chromatin accessibility in controlling regulatory DNA elements, and unravel the mechanism of gene expression regulation.
Fig.1 Different accessibility states reflect the distribution of chromatin dynamics across the genome. (Klemm, 2019)
To measure the chromatin accessibility, we provide different strategies to meet your specific requirements. Please reach out to our scientists for detailed communication about your program.
- ATAC-seq (Assay for Transposase-accessible Chromatin Using Sequencing)
Relying on the transposase-mediated insertion of sequencing primers into specific chromatin regions, ATAC-seq can provide genome-wide profiles of accessible regions of chromatin. We offer this technology to help our clients profile the active regulatory regions of chromatin.
Fig.2 ATAC-seq principal to measure the chromatin accessibility. (Klemm, 2019)
- DNase-seq (DNase I Hypersensitive Site Sequencing)
Using DNase to cleave DNA within accessible chromatin (the cleavage is greatly attenuated at the protein-binding location), accessible library fragments can be generated to evaluate the chromatin accessibility. We provide this traditional technology to advance your chromatin accessibility research.
Fig.3 DNase-seq principal to measure the chromatin accessibility. (Klemm, 2019)
- MNase-seq (Micrococcal Nuclease Sequencing)
MNase-seq uses MNase to cleave and eliminate accessible DNA. We are dedicated to providing MNase-seq approach to measure the chromatin accessibility through the sensitivity to MNase digestion.
Fig.4 MNase-seq principal to measure the chromatin accessibility. (Klemm, 2019)
- NOMe-seq ( Nucleosome Occupancy and Methylome Sequencing)
Chromatin accessibility is measured using a GpC methyltransferase. Accessible DNA is methylated and nonmethylated DNA is sequenced, providing a single-molecule measure of accessibility. With extensive expertise in epigenomic services and DNA sequencing services, Creative Biolabs is committed to providing the most accurate data about chromatin accessibility.
Fig.5 NOMe-seq principal to measure the chromatin accessibility. (Klemm, 2019)
Highlight Features
- Superior data quality;
- Dedicated Ph.D. scientists;
- Comprehensive bioinformatics for chromatin accessibility;
- Industry-leading turnaround time.
As a leading international partner of the scientific research institutes and biopharmaceutical companies, Creative Biolabs has accumulated extensive experiences in epigenomic sequencing services. We are dedicated to helping our clients advance the progress and accelerate the success of your programs. If you are interested in our services, please feel free to contact us.
References
- Klemm, Sandy L., Zohar Shipony, and William J. Greenleaf. "Chromatin accessibility and the regulatory epigenome." Nature Reviews Genetics 20.4 (2019): 207-220.
- Tsompana, Maria, and Michael J. Buck. "Chromatin accessibility: a window into the genome." Epigenetics & chromatin 7.1 (2014): 33.
- Whole Genome Bisulfite Sequencing (WGBS) Service
- Reduced-representation Bisulfite Sequencing (RRBS) Service
- Targeted Methylation Sequencing Service
- Chromatin Immunoprecipitation (ChIP) Sequencing Service
- Epigenomic Sequencing Service for Formalin-Fixed Paraffin-Embedded (FFPE) Tissue
