Epigenomic Sequencing Service for Formalin-Fixed Paraffin-Embedded (FFPE) Tissue
By combining experienced epigenetic solutions, streamlined process improvement and cutting-edge technologies, Creative Biolabs provides integrated, science-driven epigenomic sequencing services to achieve cancer discovery goals using formalin-fixed paraffin-embedded (FFPE) tissues. We are dedicated to offering rigorous scientific expertise to ensure the most accurate and high quality results.
Background
DNA methylation is one of the most important epigenomic types, since the DNA methylation patterns are highly associated with the genomic instability and expression level of tumor oncogenes, resulting in tumor transformation, growth promotion and metastasis. In addition, DNA methylation changes in cancer samples has been identified to be the tumor biomarkers for early detection, stage and prognosis evaluation in clinical settings. Furthermore, it has been verified that DNA methylation patterns are important in mediating cancer drug resistance or sensitivity. Thus, the in-depth study of cancer epigenomics can provide clues for cancer initiation and progression, as well as potential cancer treatments. Most well-annotated clinical samples are saved as FFPE tissues. However, the DNA in FFPE tissue is damaged and fragmented because of the fixation and storage method. To address this limitation, our scientists have established advanced platforms to perform epigenomic sequencing services for FFPE tissues.
Our Epigenomic Sequencing Service for Formalin-Fixed Paraffin-Embedded (FFPE) Tissue
To improve the poor-quality DNA of FFPE tissue, our scientists have developed specific DNA restoration method to ensure the DNA quality and amount. We are committed to providing robust, accurate and reproducible results with FFPE tissue-derived DNA, which are comparable to those obtained from fresh frozen (FF) tissue. Our epigenomic sequencing services for FFPE tissues include whole genome-wide DNA methylation sequencing and ChIP sequencing.
Fig.1 Workflow of FFPE-seq service.
Highlight features
- Advanced DNA restoration platform;
- Advanced speed and precision while ensuring high quality outcomes;
- A value-added CRO with a focus on epigenomic sequencing services;
- Uncompromising commitment to one-stop epigenomic solutions.
At Creative Biolabs, our scientists not only work with their minds, but also put their passion into the programs. We are glad to take challenges to accomplish various epigenomic sequencing programs. With senior scientists and more than 10 years’ experiences, we are committed to facilitating the success of your programs leveraging our full group-wide potential. Please contact us for more details.
References
- De Ruijter, Tim C., et al. "Formalin-fixed, paraffin-embedded (FFPE) tissue epigenomics using Infinium HumanMethylation450 BeadChip assays." Laboratory investigation 95.7 (2015): 833.
- Dumenil, Troy D., et al. "Genome-wide DNA methylation analysis of formalin-fixed paraffin embedded colorectal cancer tissue." Genes, Chromosomes and Cancer 53.7 (2014): 537-548.
- Amatori, Stefano, et al. "Epigenomic profiling of archived FFPE tissues by enhanced PAT-ChIP (EPAT-ChIP) technology." Clinical epigenetics 10.1 (2018): 143.
- Whole Genome Bisulfite Sequencing (WGBS) Service
- Reduced-representation Bisulfite Sequencing (RRBS) Service
- Targeted Methylation Sequencing Service
- Chromatin Immunoprecipitation (ChIP) Sequencing Service
- Chromatin Accessibility Sequencing Service
