Whole Genome Bisulfite Sequencing (WGBS) Service

Methylation of DNA at the fifth position in cytosine (5-mC) is a typical epigenetic modification that involves a variety of biological processes, including gene silencing, genomic imprinting, and X chromosome inactivation. Increasing evidence has proved DNA methylation is associated with cancer, and abnormal hypermethylation is commonly found in tumor suppressor gene promoter CpG islands during the development of cancer. Hence, detection and quantification of DNA methylation are critical to understand tumorigenesis.

Creative Biolabs is a world-leading technology service company who has accumulated rich experience in cancer research based on next-generation sequencing (NGS) technology. Leveraging our expertise in DNA methylation profiling and advanced technical platform, we are very honored to offer the superior-quality whole genome bisulfite sequencing (WGBS) services for global customers, enabling to identify individually methylated cytosines at a genome-wide scale.

Introduction of WGBS

WGBS combines bisulfite conversion with NGS technology, enabling to efficiently detect genome-wide DNA methylation at the single-base resolution level. All unmethylated cytosines are converted into uracil while methylated cytosines remain unchanged after the treatment of genomic DNA with bisulfite. Then NGS technology is applied to sequence the whole genome after bisulfite treatment, and the methylation of whole genome is identified at single-base resolution by comparing with the reference genome. This method is a valuable research tool and widely applied to study gene regulation, cell differentiation, embryogenesis, aging, and tumorigenesis.

Fig. 1 General principle of WGBS. (Masser, 2018)

Workflow

Features

• High accuracy: strict quality procedures to ensure the accurate analysis of each C base methylation state
• High reliability: direct sequencing and quantification of methylated fragments without cross-reaction and background noise
• Genome coverage: genome-wide methylation identification at single-base resolution
• Bioinformatics: raw data quality control, read mapping, genome-wide methylation analysis, and differential methylation analysis
• Sequencing: sequencing 2x150 bp on Illumina platform, sequencing depth ≥ 30X, Q30 sequencing score > 80%
• End-to-End services: from sample preparation to data analysis
• Rapid turnaround time: 4-8 weeks

Sample Requirements

• DNA amount: ≥ 4.0 μg
• DNA concentration: ≥ 50 ng/μl
• DNA volume: ≥ 20 μl
• Purity: OD260/280=1.8~2.0, without degradation, no RNA or protein contamination

Creative Biolabs has accomplished several challenging WGBS projects and accumulated rich experience in methylation analysis. We are confident in offering the first-in-class WGBS services to help you to detect genome-wide DNA methylation at single-base resolution. Besides, our excellent expert team strictly follows quality procedures to ensure accurate data delivery. Please do not hesitate to contact us for more information and a detailed quote.

Reference

1. Masser, D.R.; et al. Analysis of DNA modifications in aging research. GeroScience. 2018, 40(1):11-29.

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