Targeted Methylation Sequencing Service
With unrivaled expertise and experiences in epigenomics services, Creative Biolabs independently developed a novel targeted methylation sequencing platform to analyze the DNA methylation for the target regions of interest. As expert in DNA methylation analysis, our scientists are dedicated to one-stop solutions from sample preparation to data analysis to meet your specific requirements and budgets.
Background
Epigenetic processes play critical roles in the control of gene expression, and DNA methylation at specific regions have been identified as the dynamic and long lasting regulator of gene expressions. With the development of next-generation sequencing (NGS) technologies, epigenomics has been investigated and explored through the approaches including whole genome bisulfite sequencing (WGBS), reduced-representation bisulfite sequencing (RRBS), chromatin accessibility sequencing etc. To analyze the cytosine methylation at precise locations throughout the genome of interest, such as specific gene promoters or CpG islands, we established advanced targeted methylation sequencing platform, providing high-throughput, quantitatively accurate and publication-ready results by our bioinformatics scientists.
Our Targeted Methylation Sequencing Service
Leveraging the power of our SuPrecision™ platform, we offer targeted methylation sequencing service to our clients, facilitating your cancer research about gene expression and chromatin remodeling. We are dedicated to providing insights into targeted methylation patterns through approaches such as amplicon methyl-seq and target enrichment, at a single nucleotide level for specific regions of interest. We provide end-to-end targeted methylation sequencing services including primer desing and validation, targeted amplification, adapterization and barcoding, sequencing, sequence alignment and DNA methylation analysis. With extensive experiences and advanced platforms, we are committed to following strict quality procedures and delivering comprehensive and accurate results.
Fig.1 Workflow of targeted methylation sequencing service.
Advantages
- Reduced sequencing costs compared with whole genome methylation sequencing
- Profiling DNA methylation in specific regions of interest
- Increasing sequence coverage of CpG islands
Featured Highlights
- End-to-end solutions from sample preparation to data analysis
- Rapid turnaround time
- High quality raw data and methylation analysis
- High sequencing depth
Empowered by our targeted methylation sequencing platform, Creative Biolabs is dedicated to facilitating our clients’ researches for studies on development, cell differentiation, cell cycle, DNA repair as well as genomic imprinting. We offer comprehensive services including sample preparation, DNA extraction, bisulfite modification, library preparation, high throughput sequencing, data analysis and assay validation etc. We are committed to providing new avenues to advance your cancer epigenomics research. Please feel free to contact us to learn how we can be involved in your project.
References
- Masser, Dustin R., David R. Stanford, and Willard M. Freeman. "Targeted DNA methylation analysis by next-generation sequencing." JoVE (Journal of Visualized Experiments) 96 (2015): e52488.
- Ziller, Michael J., et al. "Targeted bisulfite sequencing of the dynamic DNA methylome." Epigenetics & chromatin 9.1 (2016): 55.
- Whole Genome Bisulfite Sequencing (WGBS) Service
- Reduced-representation Bisulfite Sequencing (RRBS) Service
- Chromatin Immunoprecipitation (ChIP) Sequencing Service
- Chromatin Accessibility Sequencing Service
- Epigenomic Sequencing Service for Formalin-Fixed Paraffin-Embedded (FFPE) Tissue
