Mouse Anti-ABO Monoclonal Antibody (AGM-006YJ) (CAT#: AGM-006YJ)
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Datasheets
Specifications
| Target | ABO |
| Species Reactivity | Human |
| Host | Mouse |
| Immunogen | ABO (NP_065202.2, aa 273-354) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Antibody Isotype | IgG1 |
| Application | WB, ELISA |
| Conjugate | Unconjugated |
| Format | Liquid |
| Size | 100 μg |
| Buffer | PBS, pH 7.4 |
| Storage | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or below long term. Avoid multiple freeze-thaw cycles. |
Target
| Alternative Names | Blood Group B Transferase/GTB/ABO; ABO; GTB; A transferase; A3GALNT; A3GALT1; ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase;transferase B, alpha 1-3-galactosyltransferase); ABO glycosyltransferase; ABO; B transferase; B(A) alpha-1,3-galactosyltransferase; EC 2.4.1.37; EC 2.4.1.40; Fucosylglycoprotein 3-alpha-galactosyltransferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; Glycoprotein-fucosylgalactoside alpha-galactosyltransferase; Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase; GTB; Histo-blood group A transferase; histo-blood group A2 transferase; histo-blood group ABO system transferase; Histo-blood group B transferase; NAGAT |
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Q: Can this antibody be used for therapeutic purposes?
A: No, the Mouse Anti-ABO Monoclonal Antibody is designed for research use only and is not intended for therapeutic applications.
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Q: What species does the Mouse Anti-ABO Monoclonal Antibody react with?
A: This antibody specifically reacts with human ABO blood group antigens, making it suitable for studies involving human samples.
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Q: How is the antibody's specificity verified?
A: The specificity of the Mouse Anti-ABO Monoclonal Antibody is verified through rigorous testing, including ELISA, flow cytometry, and immunohistochemistry, to ensure it accurately binds to ABO antigens without cross-reactivity.
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Q: What is the concentration of the Mouse Anti-ABO Monoclonal Antibody provided?
A: The antibody is supplied at a concentration of 1 mg/mL, which is suitable for a variety of experimental applications.
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Q: Are there any known limitations or precautions when using this antibody?
A: Yes, it is important to follow the recommended protocols and avoid using the antibody in conditions it is not validated for, such as in vivo studies or therapeutic use. Always validate its performance in your specific application.
Kidney transplant biopsy 12 weeks after ABO-incompatible kidney transplantation.
The case report discusses a severe antibody-mediated rejection (AMR) after ABO-incompatible kidney transplantation complicated by Serratia marcescens sepsis. A 58-year-old woman received an ABO-incompatible transplant, with initial anti-A titers reduced to safe levels. However, 12 weeks post-transplant, her anti-A IgM titers spiked to over 5000 following sepsis, leading to fulminant AMR. Experiments hypothesized bacterial epitopes shared with blood group A antigens, although direct interaction was not demonstrated.
The significance of this research lies in highlighting the potential for Gram-negative infections to trigger late AMR in ABO-incompatible transplants. The findings suggest that while ABO-incompatible kidney transplantation can be managed effectively with current desensitization protocols, post-transplant infections remain a critical risk factor for severe rejection episodes. This case underscores the need for vigilant post-transplant monitoring and rapid intervention during infections to prevent antibody-mediated complications
de Weerd, Annelies et al. "Late antibody-mediated rejection after ABO-incompatible kidney transplantation during Gram-negative sepsis." BMC nephrology vol. 15 31. 12 Feb. 2014, doi:10.1186/1471-2369-15-31
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Specificity
This antibody provided excellent specificity in our flow cytometry assays, yielding clear and distinct ABO antigen staining. It's a valuable addition to our toolkit. -
Sensitivity
The Mouse Anti-ABO Monoclonal Antibody has significantly enhanced our ELISA results. Its high sensitivity and specificity make it a top choice for ABO antigen detection. -
Accuracy
In our immunohistochemistry studies, this antibody consistently produces precise and accurate ABO antigen staining. It's a dependable reagent for histological research. -
Stability
This antibody has been crucial for accurate ABO typing in our human sample analyses. The stability and reliability over extended use are outstanding. -
Customer Support
The customer support team provided excellent assistance, addressing all our queries promptly and efficiently. Their guidance ensured we achieved the best results with this antibody.
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