T Cell based RNA/DNA Detection Assay Service
Creative Biolabs provides a direct solution to the long-standing challenges of molecular diagnostics. By leveraging the natural signal amplification of a T cell's activation cascade, our service can detect nucleic acid targets at concentrations far below the limits of conventional PCR and next-generation sequencing (NGS) methods. The core deliverable is a comprehensive, high-sensitivity diagnostic report, providing not just presence/absence data, but also quantitative insights into target copy number.
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Assessing Cytokine Production by PCR
Cytokines are typically measured using ELISA, cytometric bead array, and intracellular staining methods with cell cultures or supernatants. However, in some circumstances, only a small number of cells are available, or tissue samples are applied to detect cytokines. The measurement of the mRNA expression level of cytokines is a useful approach to estimating cytokine production potency.
The PCR-based molecular technique--reverse transcription-polymerase chain reaction (RT-PCR) is adopted to amplify target genes using an mRNA template, and is of high sensitivity and specificity for detecting 6 DNA and 40 RNA copies/reaction, respectively.
For a comprehensive review of our capabilities and a detailed discussion of your project requirements, request a consultation.
Fig.1 Methods of miRNA detection and quantification by RT-qPCR.1
What We Can Offer
T-Cell Activation Profiling
We specialize in profiling cancer epitopes by precisely measuring T-cell activation-dependent cytokine production. Our method determines cytokine gene expression at the transcription level for comprehensive insights.
T-PCR Based Analysis
Utilizing the reliable RT-PCR technique, our service provides accurate and reproducible data on cytokine mRNA abundance. This approach ensures precise measurement of gene expression levels to guide your research.
Broad Cytokine Detection
Our assay is designed for versatility, allowing the detection of any cytokine of interest to support your research needs. We routinely monitor a wide range of prevalent cytokines.
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A list of the prevailing monitored cytokines
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IL-2
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IL-4
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IL-10
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IL-12
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IL-17A
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IFNg
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TNFa
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Granzyme B
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Perforin
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T Cell-based RNA/DNA Detection Assay Services at Creative Biolabs
Highlights
Superior Sensitivity
Our T Cell-based assay harnesses the natural biological amplification of a T cell's signaling cascade. This innovative approach enables single-molecule detection, providing superior sensitivity and a lower limit of detection than conventional methods.
Unmatched Specificity
The assay can recognize target nucleic acid sequences with extreme precision. This highly specific recognition mechanism minimizes background noise, ensuring accurate and reliable results in complex biological samples.
Customizable Platform
Our modular assay platform is designed for maximum adaptability to your unique project needs. We can tailor the assay to detect any RNA or DNA target of interest, making it a versatile tool for a wide range of research.
Proven Reliability
Our technology is built upon a robust foundation of published data and successful case studies. This peer-reviewed evidence and our track record of client success provide confidence in the unparalleled performance and precision of our detection platform.
Discover how a partnership with us can streamline your research - Get a quote today.
Customer Reviews
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Unmatched Sensitivity
This assay has been a game-changer, greatly improving the detection of rare viral transcripts in patient samples that were previously missed by conventional methods. The high data quality and low sample volume are invaluable. - J***s W**t.
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Higher Specificity & Speed
We found Creative Biolabs' platform offered superior specificity and a surprisingly fast turnaround compared to our in-house NGS pipeline. It provided reliable data for our liquid biopsy validation studies. - S*****a M**k.
FAQs
How does the T Cell-based assay compare to PCR in terms of sensitivity?
Our assay leverages the biological amplification of a T cell's signaling cascade, allowing it to detect targets at single-molecule resolution. This often provides significantly higher sensitivity than traditional PCR methods, especially for extremely low-abundance biomarkers.
What types of samples can you analyze?
Our platform is highly versatile and can be used with a variety of sample types, including plasma, serum, cerebral spinal fluid (CSF), urine, and tissue homogenates. We are committed to working with you to find a solution tailored to your specific sample type and research goals.
Related Services
Cell Line Development
Our comprehensive service covers the entire workflow from gene synthesis to high-throughput clone screening, ensuring the creation of high-producing, stable cell lines for antibody production.
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Biomarker Development Service
We offer end-to-end biomarker analysis services, from selection and validation to application in drug discovery. Our customized solutions use a wide range of singleplex and multiplex technologies.
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How to Contact Us
Creative Biolabs is a reliable partner for cancer epitope discovery. We have assembled a team of experts in tumor immunology and a robust PCR technical platform to provide accurate, sensitive, and specific cytokine detection services using the classical PCR method. Interested in this specific and efficient assay? Please contact us and deliver your requirements.
Reference
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Roberts, Thomas C et al. "Detection and quantification of extracellular microRNAs in murine biofluids." Biological procedures online vol. 16,1 5. 14 Mar. 2014. Distributed under an Open Access license CC BY 4.0, without modification. https://doi.org/10.1186/1480-9222-16-5
For Research Use Only | Not For Clinical Use
