Creative Biolabs provides a direct and quantitative readout of T cell function, moving beyond simple cell counts to reveal what the cells are actively producing. Our service delivers precise and actionable insights into the immune response, which is crucial for vaccine and drug development. We can help you identify and characterize rare antigen-specific T cell populations and determine their cytokine, granzyme, and perforin profiles at a single-cell level. This detailed analysis is a cornerstone for determining the efficacy of a novel therapeutic or for monitoring disease progression.
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Intracellular Staining Assay for Cancer Epitope Analysis
The intracellular staining approach is a prevailing method used to quantitatively measure the immune response-related proteins produced in cells, including cytokines and granzyme, and perforin, to assess cell responses to various stimuli. With a deep understanding of T cell immunity and successful experience in T cell phenotype and function study, Creative Biolabs offers T cell-based intracellular staining assay to study cancer epitope-induced specific T cell cytokine, granzyme, and perforin production, providing scientific information on the determination of targets for cancer immunotherapy.
Comparison of the ELISPOT Assay and Intracellular Staining Assay
ELISPOT Assay
Intracellular Staining Assay
Analysis of pools of cancer epitope peptides
Recommended for the first round of large-scale screening
A small number of cells are needed
Monitor the T-cell response to a specific peptide or a small number of peptides
Providing comprehensive information on phenotype and function
Costs a larger number of cells
To explore our solutions in detail and to arrange a consultation - Request a consultation.
What We Can Offer
We provide a comprehensive quantification service on epitope-specific intracellular molecules using intracellular staining in combination with the ELISPOT assay.
List of Cytokines and Effectors Assessed by Our Intracellular Staining Assay
IL-6
IL-2
IL-3
GM-CSF
TNFa
TNF
IL-15
IL-12
IL-8
IL-5
IL-1b
IL-13
IL-17
IFNg
Perforin
Granzyme A
Granzyme B
CCL4/MIP-1b
CCL3/MIP-1a
Intracellular Staining Assay Services at Creative Biolabs
Highlights
Enhanced Detection
Our assay leverages a protein transport inhibitor to trap effector molecules like cytokines in the rough endoplasmic reticulum, ensuring they are localized and easy to detect with high sensitivity.
Direct Crude Sample Analysis
Our optimized protocols are designed to directly handle complex, unpurified samples such as PBMCs or splenocytes, streamlining your workflow by eliminating the need for upfront cell separation.
Simultaneous Multi-Parameter Analysis
We can detect both intra- and extracellular protein markers simultaneously in every single target cell. This allows for a comprehensive, single-cell phenotypic and functional analysis.
Accuracy and Reliability
Our scientists are well-versed in the technical nuances of the assay, including potential artifacts, ensuring we deliver data that is not only robust but also scientifically accurate and reliable for your research.
Discover how a partnership with us can streamline your research - Get a quote today.
Customer Reviews
Superior to ELISPOT for complex analysis
Creative Biolabs' service provided a level of detail on T cell phenotype and cytokine co-expression that we couldn't get with ELISPOT. It was essential for our project on T cell subsets and their role in inflammation. - P. H***, Lab Director
Reliable and reproducible results
We have used the Creative Biolabs service to monitor immune responses in our animal models for over a year. Their meticulous approach to sample preparation has given us highly consistent data that is critical for our long-term studies. - J. L****y, Principal Scientist
FAQs
Can you analyze multiple cytokines at once with this assay?
Absolutely. Our state-of-the-art multi-color flow cytometry platform allows us to detect and quantify multiple cytokines and other intracellular proteins simultaneously, giving you a comprehensive functional profile of your T cells.
Is the T Cell-based intracellular staining assay suitable for my unpurified cell samples?
Yes, our optimized protocol is designed to handle crude samples like PBMCs. In fact, we take an extra step to mitigate potential artifacts caused by contaminating cells, ensuring your final data is accurate and reliable.
Related Services
T Cell Proliferation Assay Service
Measure the ability of T cells to clonally expand in response to stimulation. This service provides a complementary and equally important view of the immune response, offering crucial insights into cellular proliferation kinetics and viability.
We offer expert assistance in designing and validating custom multi-color antibody panels, ensuring optimal marker selection for your flow cytometry assays.
Creative Biolabs offers comprehensive assays to provide reliable and accurate data on cytokines and cytotoxic molecule production in activated T cells. Besides intracellular staining, we also provide the following assays based on the measurement of cytokines. Creative Biolabs is devoted to providing the most proper assays to fit your needs and ensuring high-quality reports. Please feel free to contact us to discuss your projects.
