Creative Biolabs provides advanced ELISpot assays for cancer epitope analysis, measuring cytokine secretion activity to identify antigen-specific humoral and cellular immunity.
Learn More →T & B Cell based ELISA Assay Service
Background What We Can Offer Workflow Why Choose Us FAQs Customer Review Related Services Contact Us
A most elaborate study on epitope analysis can provide detailed information on the peptides in a protein antigen directly associated with T cell recognition and antibody binding. Using the versatile ELISA assay, Creative Biolabs offers cancer epitope analysis service by detecting the cytokines released by epitope-specific immune cells.
Enzyme-linked Immunosorbent Assay (ELISA) for Cytokines Test
Cytokines are soluble proteins of polypeptides or glycoproteins that are secreted by immune cells, including neutrophils, monocytes, macrophages, B cells, and T cells at a certain period, to modulate immune responses to stimulus. Enzyme-linked immunosorbent assay is the gold standard of immunoassays in the measurement of cytokines in plasma and cell cultures. The assay is based on antigen-antibody-specific binding and enzymatic reactions conjugated to the detection antibody.
The List of T and B Cell Cytokine Analytics
We have a wide range of ELISA kits to ensure that you can choose the proper candidates for your research.
| The List of T and B Cell Cytokine Analytics | |||||||
|---|---|---|---|---|---|---|---|
| IFNg | IL-5 | GM-CSF | TNF | IL-2 | IL-10 | IL-4 | TGFβ |
| IL-6 | IFNα | IFNβ | IL-12 | IL-13 | IL-1b | IL-8 | TNFα |
| Granzyme B | IL-9 | IL-21 | IL-22 | IL-23 | IL-17 | IL-17A | Lymphotoxin A/TNFβ |
| CCL3/MIP-1a | CXCL9/MIG | CXCL10/IP-10 | CCL4/MIP-1b | Lymphotactin | |||
ELISA-based Cancer Epitope Analysis Services at Creative Biolabs
The measurement of cytokine release is an effective approach to monitoring epitope-specific T-cell and B-cell responses. Now, we are proud to introduce the most popular ELISA-based method for cancer epitope analysis.
Workflow of the ELISA assay
Work steps of sandwich ELISA
Fig.1 Protocol for sandwich ELISA. 1
- Fixed capture antibody on a solid plate
- Add cell supernatant
- Add enzyme-labeled detection antibody
- Removing unbound enzyme conjugate and added substrate
- Detect signals by spectrophotometry
Why Choose Us?
With over two decades of specialized experience, Creative Biolabs has established itself as a trusted partner in the biotech and pharmaceutical industries. Our expertise, combined with state-of-the-art technology, ensures that we provide more than just data—we provide solutions. Our T and B cell-based ELISA assay is not just a service; it's a testament to our dedication to advancing scientific discovery.
We invite you to partner with us to accelerate your research and development pipeline. Contact our expert team today to discuss how our T and B cell-based ELISA assay can be customized to meet the unique needs of your next project.
FAQs
Q1: How does your assay differ from a traditional ELISpot assay?
A1: While both are powerful assays, ELISA quantifies the total amount of cytokine secreted into the supernatant, providing a robust measure of the overall response. ELISpot, on the other hand, provides single-cell resolution by counting the number of cytokine-secreting cells.
Q2: What specific cytokines can you detect?
A2: Our platform is highly flexible and can be configured to detect a wide range of human or animal cytokines and chemokines, including but not limited to IFN-γ, IL-2, IL-4, IL-6, and TNF-α.
Q3: Can this assay be used for my specific disease model?
A3: Our assay is highly versatile and can be customized to support research in a variety of fields, including infectious diseases, autoimmune disorders, and oncology. We work closely with our clients to design the antigen stimulation and readout to meet their project's unique needs.
Customer Review
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Improved Screening for Functional Responses
Using Creative Biolabs' T and B cell-based ELISA assay in our research has significantly improved our ability to screen for functional responses to our lead compounds, providing the quantitative data we need for informed decisions. - Mk W*on -
Maximizing Use of Precious Samples
The assay's low cell requirement has been a game-changer for our work with precious clinical samples, enabling us to obtain comprehensive data from a single, small sample. - Aa M*tinez
Related Services
To further support your research, Creative Biolabs offers a suite of complementary services designed to provide a more complete picture of the immune response.
T and B Cell-based ELISPOT Assay
Chemokine Production Measurement Service
Creative Biolabs offers unparalleled chemokine production measurement services for various research needs. Leveraging advanced technology, our highly skilled scientists provide comprehensive assays to meet diverse customer project requirements.
Learn More →Contact Us
Creative Biolabs' T and B cell-based ELISA assay offers a comprehensive, high-throughput solution for characterizing cellular immune responses, enabling you to accelerate your research in vaccine development, immuno-oncology, and diagnostics. Our service is designed to provide you with fast, accurate, and actionable data that drives your discoveries forward.
Contact Our Team for More Information and to Discuss Your Project.
Reference
- Gárate, Gabriel, et al. "Untangling the mess of CGRP levels as a migraine biomarker: an in-depth literature review and analysis of our experimental experience." The Journal of Headache and Pain 25.1 (2024): 69. Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.1186/s10194-024-01769-4
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