T Cell based Cytometric Bead Array Assay Service
Background What We Can Offer Workflow Why Choose Us FAQs Customer Review Related Services Contact Us
The success of the immunotherapy is associated with the epitope-specific T-cell immune responses that are strong and broad. With decades of bioassay developing experience, Creative Biolabs offers the flow cytometric beads-based assay for discovering T cell-associated cancer epitopes.
T Cell-based Cytometric Bead Array Assay
Based on the epitope-specific cytokines release response from T cells, it is possible to pick the epitope candidates that could stimulate robust T-cell responses. Traditionally, enzyme-linked immunosorbent assay (ELISA) is used to quantitatively measure the presence of cytokines in a sample. Cytometric bead assay is a technique that melds ELISA-based technology with flow cytometry to simultaneously test multiple analytes in a small volume of sample in a single reaction.
Fig.1 Cytometric bead arrays.1
The List of Analytes in the Assay
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IL-1b
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IL-6
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IL-10
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IL-17A
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IL-23
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Granzyme A
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CCL2/MCP-1
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CXCL10/IP-10
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IL-2
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IL-7
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IL-12
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IL-17F
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IFNγ
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Granzyme B
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CCL4/MIP-1b
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IL-4
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IL-8
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IL-13
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IL-21
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TNF
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GM-CSF
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CCL5/RANTES
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IL-5
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IL-9
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IL-17
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IL-22
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TNFα
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TGFβ
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CXCL9/MIG
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In addition to the analytes above, Creative Biolabs also provides functional beads conjugated with your antibody for the antigen of your interest. We are dedicated to helping our clients meet the various demands of cancer epitope identification. If you are interested in the service, please contact us.
Cytometric Bead Array Service at Creative Biolabs
Creative Biolabs has developed flow cytometric bead-based immunoassays to evaluate epitope-specific cytokine secretion from T cells. In the assay, a mixture of fluorescent-labeled microbeads and highly specific antibodies are designed to capture cytokines, and flow cytometry is utilized to read out the results of the immunoassays.
Why Choose Us?
Creative Biolabs combines cutting-edge technology with extensive scientific expertise to offer a T cell-based cytometric bead assay service that is second to none. Our platform is a superior alternative to traditional methods due to its high sensitivity, exceptional multiplexing capabilities, and robust, quantitative results. We also offer full customization for any species or analyte panel, ensuring the service is perfectly tailored to your project.
Contact us today to learn how our services can accelerate your journey from discovery to therapeutic development.
FAQs
Q1: How does this assay compare to a traditional ELISA?
A1: While ELISA is a great tool, our cytometric bead assay offers significant advantages. It allows for the simultaneous measurement of up to 10 different analytes from a single, small sample volume, saving you valuable time and resources compared to running multiple, separate ELISA assays.
Q2: Is the cytometric bead assay quantitative?
A2: Absolutely. Our assay provides highly quantitative data, reporting cytokine concentrations in picograms per milliliter (pg/mL), which is critical for making robust, data-driven decisions in your research.
Q3: Can you develop a custom panel for my specific research needs?
A3: Yes, our platform is fully customizable. Our team of experts can develop a bespoke bead-based panel to quantify any target cytokines or biomarkers to perfectly match your project requirements.
Customer Review
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High Sensitivity
Using Creative Biolabs' assay in our research has significantly improved our ability to detect low-level cytokine signals. The sensitivity is far superior to ELISA, allowing us to capture subtle immune responses we previously missed. - Po Rz, C***x
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Customization for Animal Models
Using Creative Biolabs' assay in our research has significantly streamlined our preclinical studies on animal models. We needed a custom assay for bovine cytokines, and their team was able to develop a reliable and validated panel, which was a huge advantage over finding a commercial kit. – Se*Wh
Related Services
To help you achieve your research goals, we recommend these complementary services:
T Cell-based Reporter Gene Assay
Creative Biolabs provides innovative, high-throughput reporter gene-based assays for T-cell response monitoring and cancer epitope identification. These assays provide a selective indicator of antigen-specific T-cell activation.
Learn More →
Immune Cell Surface Marker Identification Service
Creative Biolabs offers immune cell surface marker identification services to distinguish different immune cells and accelerate immunology research. These markers, including membrane antigens and receptors, are crucial for evaluating cell number, function, and phenotypic changes.
Learn More →
Contact Us
The T cell-based cytometric bead array assay is an essential technology for any researcher seeking to understand and quantify T cell-mediated immune responses. At Creative Biolabs, we combine this powerful technology with our extensive expertise in immunology to provide a comprehensive and reliable service. Our dedicated team is committed to delivering high-quality, actionable data to help you achieve your research goals.
To learn more about how our T cell-based cytometric bead assay services can accelerate your journey from discovery to therapeutic development, please reach out to us.
Reference
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Ayala-Balboa, Julio, and María C. Jiménez-Martínez. "Allergen-based diagnostic: Novel and old methodologies with new approaches." Allergen (2017): 77. Distributed under Open Access license CC BY 3.0, without modification. https://doi.org/10.1002/clt2.12292
