As a pioneer and undisputed global leader in the field of sdAb development, Creative Biolabs is professional in applying advanced immunization strategies to satisfy various project demands. Based on our extensive experience, we have won great reputation among our worldwide clients for successfully developing a series of high-quality sdAbs against various challenging targets, such as small molecules, membrane proteins, and PTM-modified targets.
Based on the target of interest and the specific project objective, our scientists could tailor the best-fit strategies to set up an appropriate immunization program. Such program will involve one of the following typical strategies, or combine some of them as a heterologous strategy, which can contribute to triggering the best immune response.
Multiple Sites Immunization
Multiple sites immunization is the most standard strategy for large animal immunization. This strategy can elicit responses from several easily accessible sites thus contribute to the development of antibodies with high specificity and affinity. It is also the best option if injection of more than one antigen is required.
Typical Advantages of Multiple Sites Immunization
- Trigger desired immune response with less amount of antigen
- Different antigens can be involved for a single immunization round
- Compatible with various antigen formats
DNA Immunization
DNA immunization is an efficient approach to elicit an immune response against challenging antigens. Compared with the traditional immunization methods, DNA immunization allows the antigen production in vivo, so that avoids the time-consuming processes of antigen preparation. What’s more, the naïve structure of the expressed antigens also permits the generation of antibodies with high specificity and affinity.
Typical Advantages of DNA Immunization
- Available for challenging antigens, such as insoluble proteins, multi-pass membrane proteins, secreted proteins, intracellular proteins, unstable proteins, etc.
- Time- and labor-saving
- Avoid the risk of antigen contamination
- Antibody generation with high affinity and specificity
Whole Cell Immunization
Whole cell immunization is a novel strategy to elicit an immune response against the antigen overexpressing cell line. Different from the conventional immunization strategies, whole cell immunization benefits from higher immunogenicity, naïve antigen structure, simple purification process. It is one of the best ways to discover novel sdAbs against the extracellular domain of desired membrane protein target.
Typical Advantages of Whole Cell Immunization
- Discover antibodies against the extracellular domain of membrane protein
- A powerful tool for the identification of new biomarkers from the surface proteins of tumor cell lines
- Novel antibodies recognize the naïve conformation of a target
Along with over a decade of extensive experience in providing immune-based sdAb discovery projects, Creative Biolabs contributed to discovering thousands of novel single domain antibodies for our customers all over the world. If you are interested in our services, please do not hesitate to contact us for more details.
