Creative Biolabs has extensive experience to offer various characterization services of iPSC pluripotency. Now our team of developers and technical support specialists work together to bring our customers the most high-efficient embryoid body (EB) formation and characterization service.
Based on the core properties of unlimited self-renewal and differentiation potentiality, human induced pluripotent stem cell (hiPSC) has been served as the exciting cell source for various applications such as drug discovery, regenerative medicine, and investigation of disease etiology and pathogenesis. It has been proved that the strategy of inducing pluripotency is valid in reprogramming adult somatic cells to be the embryonic-like state. However, the pluripotent capabilitiy and differentiation potential of the hiPSCs should be evaluated before their downstream regulation applications. Now Creative Biolabs provides the embryoid body (EB) formation and characterization assay to characterize pluripotency and differentiation potential for iPSC.
Fig.1 Contracting Embryoid Bodies.
The generation of embryoid bodies from iPSCs is an in vitro approach for the pluripotency evaluation. EBs are three-dimensional aggregates of cells which contain the amalgam of the three developmental germ layers. In our method, the undifferentiated hiPSCs are placed in a suspension that promotes random differentiation of cells into all three germ layers. The formation of EBs is a common approach to differentiate iPSCs into different cell types. The advantage of this approach is that the regulatory issues and extensive expenses associated with maintaining immune-deficient mice can be avoided in in vitro culture with standard tissue culture methods and materials. Our service portfolio includes various methods for the generation of EBs and subsequent analysis in biochemistry and histology, the details can be read as follow.
To assess the pluripotency of hiPSCs by in vitro methods to derive EBs, it is necessary to carry out a definitive downstream assay that demonstrates the ability to form a representative of three developmental germ layers, thereby demonstrating their increased differentiation capacity upon reprogramming.
Fig.2 Histological evidence of germ layer differentiation in embryoid bodies.
Based on our extensive experience and advanced platform, Creative Biolabs offers a series of characterization services of iPSC pluripotency. In addition, other services regarding iPSC generation and iPSC applications can also be found on our web pages. If you are interested in them, please feel free to contact us for more details.
Reference
For Research Use Only. Not For Clinical Use.
