Characterization Services of iPSC Plutipotency

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Characterization Services of iPSC Plutipotency

Induced pluripotent stem cells (iPSC) are cells derived from somatic cells with a number of defined factors. They served an important role in cell and tissue modeling, drug screening, and may also in future regenerative therapies. The characteristics of iPSC are similar to embryonic stem cells, thus similar methods are developed to characterize and differentiate them. With years of exploration in iPSC development, Creative Biolabs is dedicated to providing several characterization services of iPSC plutipotency for customers all over the world.

Cell line Characterization and Pluripotency

To characterize the plutipotency of iPSC, a class of defined tests can be used, and usually iPSC are characterized with the criteria applied to ES cells. Due to the differences between iPSC and ES cell expression patterns, their characteristics also not exactly the same. There are a variety of features assessed by characterization, such as cell and colony morphology, the expression of surface markers and other antigens, growth rate, the expression of pluripotency marker genes, enzyme levels, methylation statuses, as well as differentiation into somatic cell types in vitro and in vivo. Besides, among numerous pluripotency markers, OCT3/4 and Nanog are considered to be archetypal pluripotent stem cell markers. Moreover, it has demonstrated that OCT3/4 and SOX2 work together via feedback loops regulating both their own transcription and other pluripotency-associated genes.

Immunofluorescence staining for pluripotency markers. (Lampuoti, J. 2013) Fig 1. Immunofluorescence staining for pluripotency markers. (Lampuoti, J. 2013)

Methods for iPSC Pluripotency Characterization

Creative Biolabs is dedicated to providing several viable and cost-effective characterization methods for iPSC plutipotency. Usually, these approaches are based on RNA extraction, cDNA transcription, polymerase chain reaction, and immunofluorescence staining. Based on our well established iPSC platform, we are confident in offer the first class characterization services to contribute greatly to your project's success. Our services include but are not limited to items.

Markers to distinguish pluripotent and non-pluripotent cells now available for pluripotency marker assays in Creative Biolabs are showing in table 1.

Table 1. Gene and antigen markers analyzed for iPSC pluripotency. (Lampuoti, J. 2013)

Marker Analyzed expression Role/type
c-Myc Gene Expression amplifier, reinforcing the pluripotent state
Nanog Gene, antigen Establishment of induced pluripotency, resisting differentiation
OCT3/4 Gene, antigen Pluripotency maintentance and induction
Rex1 Gene Transcription factor (zinc finger protein), regulated by OCT3/4, SOX2, & Nanog
SOX2 Gene, antigen Pluripotency maintentance and induction
SSEA4 Antigen Stage-specific embryonic antigen, cell surface glycolipid
TRA 1-60 Antigen Stem cell surface keratan sulfate antigen epitope
TRA 1-81 Antigen Stem cell surface keratan sulfate antigen epitope

With professional scientists devoted themselves in iPSC Pluripotency Characterization, Creative Biolabs is dedicated to providing the first class characterization services of iPSC plutipotency for our customers. Please contact us for more information and a detailed quote.

Reference

  1. Lampuoti, J. (2013). “Characterization of Human Induced Pluripotent Stem Cells.”