Based on our extensive experience and advanced platform, Creative Biolabs has been a long-term expert in the field of iPSC technology. Now we provide the morphological analysis service for iPSCs during induced differentiation and reverse programming.
Obtained through the reprogramming of adult or fetal differentiated somatic cells by ectopic expression of a set of core pluripotency-related transcription factors (OCT4, SOX2, KLF4, and MYC), iPSCs present great therapeutic potential both in biological research and drug discovery. However, a fundamental problem concerning different degrees of induced pluripotency during the reprogramming process arises. At present, the pluripotency characteristics of human IPSCs are evaluated by pluripotency-associated cell surface markers according to molecular criteria and functional aspect by teratoma assays. In order to evaluate the morphological evolution of cells, an analysis is essential to obtain the fine structure of human iPSCs compared with human embryonic stem cells (hESCs) and differentiated cells. Now we are able to analyze the morphology of iPSC by confocal microscopy.
Fig.1 iPSC morphology in different culture conditions.
During our assay, there are three cell types have been used：human iPSCs, human ESCs, and mesenchymal stem cells (MSCs). Human ESCs can differentiate into MSCs. In this case, we are able to determine the ultrastructural characteristics of human iPSCs compared with their differentiated state in the same culture condition based on our reversible differentiation methodology. When cultured in presence of MEFs, we have found that the particular characteristics and morphological organization of the iPSC colonies are similar to those of inner mass cells at the human blastocyst stage of development, mouse embryoid bodies, and human ESCs. Moreover, we have found that the colonies contact with the culture condition via the microvillar side of the epithelial cells and there are large intercellular spaces and internal ducts to allow the transport of medium.
With our well-established iPSC platform, Creative Biolabs is dedicated to helping you with your special project. We are confident in offering iPSCs morphology analysis service for our customers all over the world. On the basis of provided cells, we will start with the cell culture and finally obtain the results through electron microscopy. Creative Biolabs also provides other various services regarding iPSC generation and applications. Please feel free to contact us for more details.
Reference
For Research Use Only. Not For Clinical Use.
