As a leading service provider that focuses on antibody development, Creative Biolabs is capable of offering a package of antibody preparation services for research, diagnostic and therapeutic use. Here, we are happy to introduce our high-quality anti-glycan monoclonal antibody development service for global clients.
Monoclonal antibodies (mAbs) are amongst the most widely used and powerful tools available to biology and medicine and their production exploits the inherent capacity of mammalian immune systems to discriminate self from non-self. To produce mAbs, animals are immunized and antibody-producing splenocytes (e.g. B cells) are removed and subsequently fused with myeloma cells. After cell fusion, large numbers of subclones are obtained and screened on the basis of antigen specificity. Once candidate hybridoma cell lines are identified, a series of functional assays will be conducted and candidate hybridoma cells will be scaled up. Until now, thousands of mAbs have been obtained and characterized by multiple applications. Particularly, anti-carbohydrate monoclonal antibodies have been identified as ideal tumor inhibitions as carbohydrate antigens overexpressed in a variety of cancers.
Fig.1 Production of monoclonal antibody. (Abi-Ghanem, 2015)
Many studies have shown that cell surface glycans play a key role in a variety of immune functions. To understand those functions, it is important to produce anti-carbohydrate antibodies and define which glycans are expressed on a cell surface at a given place and time. However, the production of monoclonal antibodies against carbohydrate is problematic as many different types of carbohydrates are intrinsically expressed in animals. To overcome this problem, Creative Biolabs has developed an advanced platform that enables us to offer various anti-carbohydrate monoclonal antibodies for global customers using our phage display or hybridoma technology. Based on our top technology, our anti-carbohydrate antibodies could recognize both glycolipids and glycoproteins. Furthermore, these antibodies can be used for multiple applications, such as immunohistochemistry, cell-staining, inhibition assay for cell adhesion, flow cytometry, ELISA and other biological experiments.
With high affinity and specificity, monoclonal antibodies have been widely applied for both laboratory study and clinical therapeutic of multiple human diseases, especially malignant tumors. In order to help global researchers find more effective approaches for the treatment and diagnosis of tumors, Creative Biolabs is dedicated to producing high-quality anti-glycan monoclonal antibodies. If you are interested in our anti-glycan service, please feel to contact us for more information.
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