Creative Biolabs has long been concentrated on antibody discovering and developing. Based on our vast experience and solid technical strength, we currently provide anti-glycan antibody detection services using the enzyme-linked immunosorbent assay (ELISA). In this assay, glycans or carbohydrates are used as antigens to coat ELISA-plates, thus providing a versatile, easy, and highly sensitive method to detect and quantify the binding of anti-glycan antibodies to glycans.
ELISA, which combines the specificity of antibodies with the sensitivity of simple enzyme assays, is a highly valuable technique that is widely used in laboratory research, medical and clinical research, and quality control in industries. Especially, it is employed as a rapid and sensitive method for the detection of antibodies/antigens in different liquid biological samples. Different formats have been developed for this purpose, including competitive ELISA, sandwich ELISA, and direct ELISA.
In the field we are discussing now, ELISA can be used for serum profiling, phage library screening, and specificity measurement of anti-glycan antibodies. Due to the low binding affinity of anti-glycan antibodies towards the antigen epitopes, a multivalent presentation of the carbohydrate ligands on the plate surface is demanded. Glycans used for immobilization can be either chemically synthesized or isolated from natural sources based on specific research requirements.
Fig.1 ELISA for anti-glycan antibodies detection.
Traditionally, wells of ELISA plates are coated with specific glycan antigens via passive adsorption for antibody detection. However, direct immobilization of some carbohydrates on the plate surface is often difficult to achieve. To address this issue, various chemical strategies have been developed to conjugate the carbohydrate antigens to proteins (bovine or human serum albumin), polymers (acrylamide derivatives) and dendrimers in order to enhance the number carbohydrate epitopes on the ELISA surface and improve the sensitivity of the assay. This method is based on the identification and characterization of the glycan antigen structures. We can help with the design of the carbohydrate immobilization strategies to push forward your cutting-edge projects.
Scientists at Creative Biolabs are professional in designing custom-specific study designs to meet all kinds of project expectations and requirements. We are happy to work with you step by step so as to ensure that the most satisfactory outcome can be acquired. If you are interested in our services, contact us to discuss your requirements.
