Surface Plasmon Resonance

The detection of anti-glycan antibodies from complex biological samples requires the use of sensitive technologies. Traditionally, the enzyme-linked immunosorbent (ELISA) is used as a routine method for antibody detection and antibody-ligand binding assessment. However, it is an indirect method and the output is limited to end-point data. Therefore, label-free technologies, such as the surface plasmon resonance (SPR), are used alternatively, to detect specific anti-glycan antibodies against different glycan determinants and characterize the antibody-antigen interactions.

Introduction to Surface Plasmon Resonance

Over the last decade, SPR biosensor has emerged as a form of tagless sensing device for the detection of analytes in liquid media. Surface plasmons created through the interaction of the light with the gold atoms of the transducer surface changes with the concentration of ligands immobilized on it, allowing sensitive measurement of surface changes caused by adsorption and/or biomolecular interactions of immobilized ligands and its analyte in solution. Compared to most immunoassay techniques, such as ELISA, SPR immunosensors show advantages including:

• Shorter detection time with high specificity, sensitivity, and greater simplicity
• Allowing measurement of sequential binding events and the specificity assessment of antibodies
• Obviating the need to label either of the interacting species and the binding event is visualized in real-time

Fig.1 Glycan microarray & SPR imaging. (Park, 2013)

SPR for Anti-Glycan Antibody Detection

SPR is ideally suited for detection/screening of anti-glycan antibodies. Glycans can be immobilized on the surface for detection of glycan-specific antibodies, measurement of antibody concentration and affinity, as well as kinetic parameters of the interaction. Moreover, we can construct an SPR-glycan microarray platform from glycans of natural or synthetic origin, pure or as a mixture, for antibody profiling or biomarker discovery. The glycan microarray is composed of numerous glycan determinants, or fragments of determinants, immobilized in a spatially defined arrangement, thus allowing high-throughput assessment.

Features

Based on our state-of-the-art technology platforms and years of experience, Creative Biolabs now offers anti-glycan antibody development services, anti-glycan antibody engineering services, and anti-glycan antibody detection services. Our services are characterized by:

• Highly experienced scientists and staff that work closely with our clients to provide in-depth scientific & technical support
• Streamlined services from consultation and project design, to product production and data interpretation
• Ensured comprehensive and reproducible results, and timeliness of delivery

Creative Biolabs is confident in offering the best and most suitable services of anti-glycan antibody services to worldwide clients. For more detailed information, please feel free to contact us or directly sent us an inquiry.

Reference:

1. Park, S.; et al. Carbohydrate microarrays. Chemical Society Reviews. 2013, 42(10): 4310-4326.