sdAb Thermodynamic Stability Improvement Service
To meet the growing demands for stability improvement of sdAbs, Creative Biolabs has established suitable solutions to improve sdAb thermodynamic stability through a variety of strategies for our clients all over the world. With advanced platforms and powerful technologies, we are confident in achieving the best outcome for our customers in a high-quality manner.
sdAbs have the potential to provide recognition reagents that combine specificity and affinity with high stability. Unlike conventional antibodies, which generally lose binding ability upon heat denaturation due to irreversible aggregation, sdAbs exhibit the capacity to refold and bind antigen after heat denaturation. Because of their thermodynamic stability, sdAbs are being exploited for a number of applications ranging from therapeutics and diagnostics to biotechnology.
sdAb Thermodynamic Stability Improvement Available at Creative Biolabs
Active in the development and manufacturing of sdAbs, Creative Biolabs is considered as a global provider to offer timely, high-quality sdAb related services for our customers all over the world. Improving the thermodynamic stability of sdAbs is vital to their successful application both as detection biomolecules and as therapeutics. While many sdAbs already exhibit refolding ability as well as elevated melting temperature (Tm), ranging from below 50°C to over 80°C, we still seek to offer custom stable sdAbs to further improve their thermodynamic stability according to our customers' specific requirements.
Fig.1 Introduction of additional disulfide bridges to stabilize the folded structure. (Hussack, 2011)
In doing so, Creative Biolabs performs rational engineering of sdAbs to increase thermodynamic stability by multiple strategies, for example:
- DNA shuffling or random mutagenesis
- Affinity selection by stringent temperature pressure
- Introduction of additional disulfide bridges to stabilize the folded structure
We will perform screening and engineering of sdAbs to increase their Tm and refolding ability, which enables them to function even after exposure to high temperatures. Utilizing our various strategies, stable sdAbs without significantly compromising their affinity will be provided, thereby providing superior reagents for research, diagnostic, and therapeutic applications.
Meanwhile, our sdAb thermodynamic stability improvement service is flexible and available to be tailored to our clients’ specific needs. Besides our sdAb thermodynamic stability improvement, we also offer sdAb proteolytic tolerance improvement service to enhance the stability of sdAbs based on specific client needs. Furthermore, Creative Biolabs offers our experience and competence in the sdAb field to find custom-made solutions to meet our customers’ other specific requirements of the stabilization of sdAb.
Along with over a decade of extensive experience, scientists at Creative Biolabs are proud to tailor the improvement of sdAb thermodynamic stability and conduct the best-fit proposal to meet your specific project requirements. Our diverse services will greatly facilitate your scientific and clinical research about sdAb. Contact us today to learn more about how we can help.
Reference
- Hussack, G.; et al. Engineered single-domain antibodies with high protease resistance and thermal stability. PloS one. 2011, 6(11), p.e28218.
Case Study
We are offering highly customized CRO services to assist your Single Domain Antibody (sdAb) related projects. Please Contact Us for more details.
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