Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsWith over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications. Highlights
Creative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsCreative Biolabs proposes contracts with guaranteed results for the hybridoma development and antibody validation for enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay (LFIA) research use. For each project, our team explores with the client the challenges of the project and defines the specifications. Our function-guaranteed antibody package will satisfy your need for quality antibodies that generate positive ELISA and/or LFIA results.
ELISA is a plate-based immunoassay for the detection of markers in various samples. Several ELISA formats are currently employed in diagnostics depending on the abundance of the target, namely direct ELISA, indirect ELISA, and sandwich ELISA. Generally, ELISA antibodies are tagged with enzymatic labels and these assays can be designed for antigen detection or quantification.
Among all ELISA formats, sandwich ELISA has become the most useful and the most challenging to develop. Sandwich ELISA employs two primary antibodies binding non-overlapping epitopes of a given antigen. Similar to all other immunoassays, the validation of antibodies for ELISA should consider the type of samples and detection conditions to be used.
While traditional PCR-based assay remains the golden standard in many areas, there is an increasing demand for other methods that enable quicker, easier, and more cost-effective tests. Double antibody sandwich-LFIA (DAS-LFIA) is an alternative strategy that can be used to detect various antigens in biological samples.
DAS-LFIA format is typically used for detecting larger molecules that have epitopes (e.g., 2 binding sites). The way they operate is quite straightforward: An antibody is attached to a binding site conjugated to a nanoparticle, and another antibody attached to another binding site is used to derive the test line. When the target molecule is present, the analyte will bind to both the antibody attached to the nanoparticle and the antibody on the test line, deriving a visual signal.
Both DAS-LFIA and ELISA require a pair of capture antibodies and detection antibodies that bind to different epitopes. However, the antibody pair with good performance in ELISA may not be effective in the LFIA detection system, and vice versa.
The majority of hybridoma projects use indirect ELISA to screen hybridomas after HAT selection. However, ELISA-guaranteed antibody generally refers to two antibodies that can be used in a sandwich ELISA or two antibodies that bind different epitopes. Selecting a pair of capture antibodies and detection antibodies is very technical. Only pairs with high specificity, selectivity, and consistent linearity of dilution for the target will be used for further development. Plasma, serum, or tissue lysate will be used to validate antibody selectivity. In many cases, purified recombinant target proteins will be added into the biological matrix, which should be recovered and detected. The recovery observed for the spike should be almost identical in both the biological matrix and the standard diluent for a sample matrix to be considered valid for our ELISA assay. The development of LFIA functional antibodies generally involves the use of a chromatography platform to verify the function of the antibody after hybridoma is subcloned and cryopreserved and antibody is purified.
Fig.1 Hybridoma development services workflow.
Let our team of antibody production experts help you reach your custom hybridoma goals. Creative Biolabs provides the services you need to help you develop a novel polyclonal antibody, monoclonal hybridoma, or recombinant antibody. We deliver exceptional antibody development solutions from antigen design to purification and screening with guaranteed results.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
