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CreMap™ Epitope Mapping & Discovery Services

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An epitope, also known as antigenic determinant, is the part of an antigen that is recognized by the immune system, specifically by antibodies, B cells or T cells. It is of great significance to get a deep understanding of protein epitopes for the disease treatment or prevention. On the one hand, scientists are trying to engineer protein or antibody drugs with reduced or eliminated immunogenicity for their effective clinical application; on the other hand, artificial peptide vaccines with the desired immunogenicity could be designed based on the epitope maps.

With the continuous development of immunology and bioinformatics, the techniques used to discover and map T cell epitopes and B cell epitopes have been greatly improved. However, problems of complicated experimental process, high economic cost, and low resolution still remain unsolved.

Creative Biolabs' excellent academic group has developed the CreMap™ T cell (or B cell) epitope discovery platform based on the expert knowledge and rich research experience in this field. We always provide professional technical services to reduce the project cost and at the same time improve the experimental accuracy.

CreMap™ T Cell Epitope Discovery Platform

CreMap™ T cell epitope discovery platform is an advanced technology for the analysis of potential T cell epitopes developed by the Creative Biolabs’ team. Compared with other strategies that require a significant investment of time and money, this platform provides an efficient and at the same time cost-effective solution which could be used for the mapping of both CD4+ and CD8+ T cell epitopes in the field of protein drug development or peptide vaccine design. Below is a brief description of the workflow of our CreMap™ T cell epitope discovery service. Read more…

Step 1: Prediction & Peptide Synthesis

An overlap peptide library is firstly constructed in silico based on the target protein sequence. Bioinformatics strategies are then used to screen out the peptides with desirable binding affinity to the specified MHC molecules. Selected peptides (the “hits”) are then synthesized for in vitro test.

Step 2: MHC-Peptide Binding Assay

Creative Biolabs will use optimized experimental methods to determine the binding kinetics between the peptide and selected MHC molecule in vitro, under the positive control of known T cell epitope. Candidate peptides with high in vitro affinity to MHC then could be identified from the hits.

Step 3: Epitope Validation

Following the in vitro MHC-peptide binding assays to identify candidate epitopes, flow cytometry combined with peptide-MHC tetramer staining would be performed to allow for the quick identification and characterization of the antigen-specific T cells Other methods such as ELISpot, intracellular cytokine staining and T cell proliferation could also be used for the epitope mapping.

CreMap™ B Cell Epitope Mapping Platform

The B cell epitope is the specific piece of the antigen to which an antibody binds. Based on their structure and interaction with the paratope of antibody, the epitopes are divided into two categories, conformational epitopes and linear epitopes. A conformational epitope is composed of discontinuous sections of the antigen's amino acid sequence. These epitopes interact with the paratope based on the 3D surface features and shape or tertiary structure of the antigen. By contrast, a linear epitope is formed by a continuous sequence of amino acids from the antigen and therefore interact with the paratope based on their primary structure.

Our expert group in Creative Biolabs has developed an advanced B cell epitope mapping platform, both conformational and linear epitopes could be effectively mapped in high-throughput and low cost. Read more…

In Silico Epitope Prediction

By using bioinformatics, Creative Biolabs provides a fast and preliminary B cell epitopes prediction of the target sequence, which could save your cost and time greatly. To ensure the accuracy, the performance of our in silico prediction method has been benchmarked against a huge number of data sets containing known binding epitopes. And both sequence-based and structure-based prediction strategies are available for your choice.

In Vitro Epitope Mapping

Our platform provides several methods for mapping B cell epitopes on the target antigens, such as X-ray co-crystallography, array-based oligo-peptide scanning, high-throughput mutagenesis mapping (alanine scanning mutagenesis), and crosslinking coupled mass spectrometry, etc.

According to your project objectives, an optimized experimental procedure will be customized to ensure a reliable result.

Fig.1 Predicted B cell epitopes of Human papillomavirus type 16 major late capsid protein L1. Fig.1 Predicted B cell epitopes of Human papillomavirus type 16 major late capsid protein L1.

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