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Phage Display & Antibody Library Services

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Creative Biolabs is one of the well-recognized experts who is professional in applying various phage display technologies to offer library construction and screening related services for a broad range of project objectives. Particularly, our services also involve specific antibody discovery (e.g. PTM-specific antibody, anti-idiotype antibody, and agonistic antibody) and peptidome discovery.

Library Construction and Screening

Library Construction and Screening
Fig.1 Workflow of phage display construction
and screening (Díez et al. 2015)

Phage display is one of the most powerful and widely used laboratory technique for the study of protein-protein, protein-peptide and protein-DNA interactions. This technology is mainly based on displaying the interest protein (peptides, antibodies, scaffolds or others) on the surface of employing phage and then be used to interrogate the constructed libraries containing millions or even billions of displayed phages. Theoretically, phage display is an exogenous gene expression method which the gene encoding the interest protein is inserted into bacteriophage coat protein gene then displaying the interest protein on the phage surfaces, resulting in a connection between genotype and phenotype.

Due to the different properties and advantages, Creative Biolabs is pleased to tailor the most appropriate phage display system (M13, T4 or T7) to meet our customers’ demands. Through DNA manipulation, numerous gene variants can be created and constructed as phage display library. Our scientists are confident in generating high-quality libraries with the diversity of 108. Furthermore, to detect the interaction between displayed protein and those other molecules or isolate specific target binders, Creative Biolabs also utilizes the novel biopanning strategies to select high-affinity binders or ligands which are able to recognize the naïve targets.

Antibody Phage Display Library
  • Immune, naïve or synthetic library construction
  • Pre-made library screening
  • Large library capacity from 107 to over 1012
  • High affinity from 10-7 to 10-9
  • Various phage display systems (M13, T4, T7)
  • Tailored biopanning strategies
  • Comprehensive QC validation

Antibody Phage Display Library

Antibody libraries are constructed by the genomic information coding for antibody variable domains, which can be derived from B cells of immune or naïve donors. Antibodies are the first proteins which were successfully displayed on the surface of phage by fusing the coding sequence of scFv or Fab to the coat protein. Due to the smaller size, scFv libraries are genetically more stable than Fab libraries, while Fab libraries lack the tendency to form higher molecular weight species, such as dimers and trimers, which can simplify the selection and characterization. Through binding the specific target, the interest antibody can then be eluted and retrieved.

Compared with the traditional hybridoma method, antibody phage display library has distinct advantages on discovering novel monoclonal antibodies and even the fully human antibody. Creative Biolabs is able to construct immune antibody libraries and isolate monoclonal antibodies with high specificity and affinity from a comprehensive list of species, which including but not limited to monkey, llama, camel, shark, alligator, mouse, rat, hamster, guinea pig, rabbit, chicken, dog, bovine, goat, sheep, and ferret. In addition, our scientists also possess some high-quality pre-made libraries (including human antibody libraries) for our library screening services.

Creative Biolabs has long-term devoted to the development and application of phage display technology. With years of experience, our scientists have developed several phage display based platforms and tailored hundreds of particular libraries and thousands of specific antibody products to boost our global customers’ research and project goals. We are pleased to use our extensive experience and advanced platform to offer the best service and the most qualified products to satisfy each demand from our customers.

Díez, P., Jara-Acevedo, R., González-González, M., Casado-Vela, J., Dasilva, N., Lécrevisse, Q., Bartolomé, R., Claros, J. C., González, A. and López, R. (2015) 'High-throughgput phage-display screening in array format', Enzyme and microbial technology, 79, 34-41.

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