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Magic™ Cell-free Membrane Protein Expression

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Creative Biolabs now provides high-quality membrane protein synthesis service using Magic™ cell-free protein expression system (CFPS) to meet the growing demand for protein research. With the help of experienced experts in our team, we offer our clients the most efficient and reliable protein expression service using our first-class cell-free expression system.

Membrane proteins are proteins that are attached to or associated with the biological membrane. They act as transporters, channels, and receptors to enable information transmission and materials exchange across the plasma membrane. As such, they manifest as primary targets for cellular functioning and therapeutic agents. In effort to improve the understanding of cell physiology as well as to facilitate drug discovery/development, Creative Biolabs has established the innovative cell-free system for generation of a variety of integral membrane proteins.

Cell-free Membrane Protein Expression

Due to the low abundance of membrane proteins in their natural hosts, it is necessary to overexpress these proteins in heterologous systems. Conventional cell-based systems (e.g. E. coli, yeast, and insect) can be problematic in insufficient membrane insertion, precipitation of de novo synthesized proteins or even cytotoxicity due to the extensive alterations in the host cell’s metabolism. Cell-free system, which is based on the modified lysates of various cellular components, provides a powerful machinery for in vivo transcription/translation of target proteins independent of a living cell’s integrity. According to different cellular origin, it can be divided into three types:


Generally, cell-free systems have multiple advantages in contrast to the cell-based expression:


Cell-free Membrane Protein Expression

Creative Biolabs now offers high-quality cell-free custom protein expression service to our worldwide clients. Our experienced scientists have earned great reputation for the successful production of many challenging membrane proteins, such as GPCRs, ion channels, and transporters. This detergent-free solution is extra suitable when the function/activity of target protein is very sensitive and easily compromised. We are always willing to tailor custom-oriented projects to meet diverse demands and expectations. Please feel free to inquiry us for more information.

References

  1. Schneider B, Junge F, Shirokov VA, et al. (2010) Membrane protein expression in cell-free systems. Methods Mol. Biol., 601: 165-186.
  2. Alkhalfioui F, Logez C, Bornert O, Wagner R (2011) Production of Membrane Proteins – Strategies for Expression and Isolation (Robinson AS, ed), Wiley-VCH: 1.



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